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Transforming growth factor-β boosts the functionality of human bone marrow-derived mesenchymal stromal cells.
Cell Biology International ( IF 3.3 ) Pub Date : 2020-08-04 , DOI: 10.1002/cbin.11437 Vaijayanti P Kale 1
Cell Biology International ( IF 3.3 ) Pub Date : 2020-08-04 , DOI: 10.1002/cbin.11437 Vaijayanti P Kale 1
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Transforming growth factor β1 (TGFβ1) is a negative regulator of hematopoiesis, and yet, it is frequently found at the active sites of hematopoiesis. Here, we show for the first time that bone marrow‐derived mononuclear cells (BM MNCs) secrete TGFβ1 in response to erythropoietin (EPO). We further show that human bone marrow‐derived mesenchymal stromal cells (BMSCs) briefly exposed to the conditioned medium of EPO‐primed MNCs, or purified TGFβ1, gain significantly increased hematopoiesis‐supportive ability. Mechanistically, we show that this phenomenon involves TGFβ1‐mediated activation of nitric oxide (NO) signalling pathway in the BMSCs. The data suggest that EPO‐MNC‐TGFβ1 could be one of the regulatory axes operative in the bone marrow microenvironment involved in maintaining the functionality of the resident BMSCs.
中文翻译：
转化生长因子-β可增强人骨髓间充质基质细胞的功能。
转化生长因子β1（TGFβ1）是造血功能的负调节剂，但是，它经常在造血功能的活性位点被发现。在这里，我们首次展示了骨髓源性单核细胞（BM MNC）响应促红细胞生成素（EPO）分泌TGFβ1。我们进一步表明，人骨髓源间充质基质细胞（BMSCs）短暂暴露于EPO启动的MNC或纯化的TGFβ1的条件培养基中，可显着提高造血支持能力。从机制上讲，我们表明该现象涉及TGFβ1介导的BMSC中一氧化氮（NO）信号通路的激活。数据表明，EPO-MNC-TGFβ1可能是在涉及维持驻留BMSCs功能的骨髓微环境中起作用的调节轴之一。
更新日期：2020-10-13
中文翻译：
转化生长因子-β可增强人骨髓间充质基质细胞的功能。
转化生长因子β1（TGFβ1）是造血功能的负调节剂，但是，它经常在造血功能的活性位点被发现。在这里，我们首次展示了骨髓源性单核细胞（BM MNC）响应促红细胞生成素（EPO）分泌TGFβ1。我们进一步表明，人骨髓源间充质基质细胞（BMSCs）短暂暴露于EPO启动的MNC或纯化的TGFβ1的条件培养基中，可显着提高造血支持能力。从机制上讲，我们表明该现象涉及TGFβ1介导的BMSC中一氧化氮（NO）信号通路的激活。数据表明，EPO-MNC-TGFβ1可能是在涉及维持驻留BMSCs功能的骨髓微环境中起作用的调节轴之一。
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