Positive effects of fermented foods consumption on humans have stimulated lots of research attention. In this study, we investigated the probiotic potentials, antagonistic activities, and safety properties of Lactobacillus brevis gp104 isolated from Iranian traditional cheese. The results showed that the strain had high resistance to acidic conditions, simulated gastric and intestinal fluid. L. brevis gp104 was able to assimilate cholesterol from the medium; 41% in medium without bile salts and 58% in medium with bile salts. The potential of this strain was relatively low in phytate hydrolyzation and 62.02% hydrophobicity, 40.2% auto-aggregation, and 48.3% co-aggregation were observed. The adhesion value of L. brevis gp104 to adenocarcinoma Caco-2 cells was 13.4% that was also confirmed by scanning electron microscopy (SEM). Antibacterial effect of L. brevis fg104 was imposed against pathogenic strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa PTCC 1707, Salmonella typhimurium PTCC 1609, and Staphylococcus aureus ATCC 25923) and the most sensitive strain S. aureus. L. brevis gp104 was able to compete (52%), inhibit (47%) and displace (21%) the adhesion of S. aureus to Caco-2 cells. L. brevis gp104 did not show haemolytic or DNase activity, which confirms its safety aspects. Therefore, L. brevis gp104 was demonstrated promising properties for its potential health benefits for its application as novel bio-therapeutic and bio-preservation agents.

发酵食品消费对人类的积极影响引起了很多研究关注。在这项研究中，我们调查了从伊朗传统奶酪中分离出的短乳杆菌gp104的益生菌潜力，拮抗活性和安全性。结果表明，该菌株对酸性条件，模拟的胃液和肠液具有较高的抵抗力。短乳杆菌gp104能够从培养基中吸收胆固醇；在不含胆汁盐的培养基中为41％，在含胆汁盐的培养基中为58％。该菌株的潜力在植酸盐水解中相对较低，并且观察到疏水性为62.02％，自动聚集为40.2％，共聚集为48.3％。短乳杆菌的附着力值腺癌Caco-2细胞的gp104为13.4％，这也通过扫描电子显微镜（SEM）确认。短乳杆菌fg104的抗菌作用是针对致病性菌株（大肠杆菌ATCC 25922，铜绿假单胞菌PTCC 1707，鼠伤寒沙门氏菌PTCC 1609和金黄色葡萄球菌ATCC 25923）和最敏感的金黄色葡萄球菌。短乳杆菌gp104能够竞争（52％），抑制（47％）和置换（21％）金黄色葡萄球菌对Caco-2细胞的粘附。短乳杆菌gp104没有显示溶血或DNase活动，从而确认其安全性。因此，短乳杆菌gp104被证明有前途的为其作为新型生物治疗和生物保鲜剂中的应用及其潜在的健康益处的属性。