This study describes the characterization of bacteriophage FP43, active against pathogenic and non-pathogenic strains of Escherichia coli, and its ability to inhibit and remove a mixed biofilm of enterohemorrhagic E. coli O157:H7 and O91:H-. Phage FP43 is a member of the Myoviridae family, having a dsDNA genome consisting of 169,248 bp with good stability. It has a short latent period and large burst size. Phage FP43 decreased the formation of biofilm, comprising E. coli O157:H7 and O91:H-, by 82.4% at 30 °C. After incubation for 6 h with FP43, viable counts of E. coli O157:H7 and total cells in the biofilm were reduced by 2.76 and 2.85 log, respectively. In planktonic cells, viable E. coli O157:H7 and total counts decreased by 3.44 and 3.62 log, respectively, after incubation with the phage for 4 h. Moreover, more than 60% of an established, mixed biofilm was removed after a 6 h exposure to the phage, in which E. coli O157:H7 and total viable counts decreased by 2.07 and 1.93 log, respectively. These results suggest that bacteriophage FP43 has broad host range against both pathogenic and non-pathogenic E. coli, and potential to reduce viable counts of both enterohemorrhagic E. coli O157:H7 and E. coli O91 in mixed-strain biofilm.

这项研究描述了对大肠杆菌的致病性和非致病性菌株具有活性的噬菌体FP43的表征，以及其抑制和去除肠出血性大肠杆菌O157：H7和O91：H-的混合生物膜的能力。噬菌体FP43是肌病毒科的成员，具有由169,248bp组成的dsDNA基因组，具有良好的稳定性。它具有较短的潜伏期和较大的突发大小。噬菌体FP43在30°C下将包括大肠杆菌O157：H7和O91：H-在内的生物膜的形成减少了82.4％。与FP43孵育6小时后，大肠杆菌O157：H7的活菌数和生物膜中的总细胞数分别减少了2.76和2.85 log。在浮游细胞中，活的大肠杆菌与噬菌体孵育4小时后，O157：H7和总计数分别降低了3.44和3.62 log。此外，在噬菌体中暴露6小时后，超过60％的已建立的混合生物膜被去除，其中大肠杆菌O157：H7和总存活数分别减少了2.07和1.93 log。这些结果表明，噬菌体FP43对致病性和非致病性大肠杆菌均具有广泛的宿主范围，并且具有降低混合菌株生物膜中肠出血性大肠杆菌O157：H7和大肠杆菌O91的活菌计数的潜力。