This study was performed to survey the incidence of Escherichia coli O157:H7 contamination in fish samples which were obtained from different fish farms and retail markets. For this purpose, a total of 140 fish samples were analyzed according to ISO 16654 and screened for virulence genes by mPCR. Antibiotic susceptibility tests were performed with the disc diffusion method and isolates were genotyped by using Enterobacterial repetitive intergenic consensus (ERIC) PCR. Of the 140 analyzed sample, two (1.4%), from the same farm, were found to be contaminated with E. coli O157 serogroup, one of which harbored stx1 and the other eaeA gene. E. coli O157 serogroup were resistant to only ciprofloxacin and were not capable of forming biofilm and their ERIC-PCR patterns were different. In conclusion, the existence of pathogenic E. coli O157 serogroup in fish samples might be a significant threat to public health and fish could serve as a vehicle for transmission of these bacteria to consumers in Turkey.

进行这项研究是为了调查从不同养鱼场和零售市场获得的鱼样品中大肠杆菌O157：H7污染的发生率。为此，根据ISO 16654对总共140个鱼样品进行了分析，并通过mPCR筛选了毒性基因。用圆盘扩散法进行抗生素敏感性试验，并通过肠细菌重复基因间共有（ERIC）PCR对分离株进行基因分型。在140份分析样本中，发现同一农场的两个样本（1.4％）被大肠杆菌O157血清群污染，其中一个含有stx1，另一个含有eaeA基因。大肠杆菌O157血清群仅对环丙沙星耐药，不能形成生物膜，其ERIC-PCR模式不同。总之，鱼样品中存在致病性大肠杆菌O157血清群可能对公共健康构成重大威胁，鱼可以作为将这些细菌传播给土耳其消费者的媒介。