Biotechnological process using xylose reductase (XR) is an ecofriendly method for production of xylitol in contrast to energy intensive chemical processes. XR is an industrially important enzyme which catalyzes the reduction of xylose into xylitol. Most of the previous data on XR producing microorganisms has been available with yeasts only. Hence, the present study on isolation of novel XR producing bacterial strains for bioconversion of xylitol was undertaken. A total number of 60 isolates was isolated from soil samples and screened for XR and xylitol production. The best xylitol producing bacterial isolate i.e. BSX-46, was characterized through polyphasic approach and found that it belongs to Pseudomonas putida. The bacterial cells were Gram negative, rod shaped, and can grow over a wide range of temperature (20 °C–37 °C), pH (5.0–7.5) and salt concentration (up to 15%, w/v). The isolate showed a very less generation time of 2 h 40 min with maximum XR activity of 48.76 ± 0.05 IU/mg of cells after 36 h of growth. This isolate has shown a good potential for the bioconversion of xylitol (35.2 g/L) with 0.98 g/L/h volumetric productivity. The results demonstrate a promising sustainable solution for xylitol production using efficient novel bacterial isolate.

与能源密集型化学过程相比，使用木糖还原酶（XR）的生物技术过程是一种生产木糖醇的环保方法。XR是一种工业上重要的酶，可催化木糖还原为木糖醇。以前有关产生XR的微生物的大多数数据仅适用于酵母。因此，进行了分离产生用于木糖醇生物转化的新型XR的细菌菌株的研究。从土壤样品中分离出总数为60的分离株，并针对XR和木糖醇生产进行了筛选。通过多相方法鉴定了产生最佳木糖醇的细菌分离株，即BSX-46，发现它属于恶臭假单胞菌。。细菌细胞呈革兰氏阴性，呈棒状，可以在很宽的温度范围（20°C–37°C），pH（5.0–7.5）和盐浓度（高达15％，w / v）中生长。分离物在生长36小时后显示的极短的2 h 40 min生成时间，最大XR活性为48.76±0.05 IU / mg细胞。该分离物已显示出以0.98 g / L / h的容积生产率进行木糖醇（35.2 g / L）生物转化的良好潜力。结果表明，使用有效的新型细菌分离物可生产木糖醇的可持续解决方案。