This study investigated the feasibility of using epigallocatechin-3-gallate (EGCG) as a carrier to interact with and precipitate peptides from soy protein hydrolysate water. EGCG was added to soy peptides, resulting in the formation of aggregates that precipitated upon centrifugation. The recovery efficiencies of peptides were evaluated at both an acidic pH of 4.5 and a neutral pH of 7.0 with varying concentrations of EGCG. Results showed that the peptide recovery increased with increasing concentrations of EGCG. The maximum recovery of peptides was 57.17%, at pH 4.5 and an EGCG/peptide ratio of 0.40. Further increases in EGCG concentrations did not result in any changes to peptide recovery, suggesting that the interaction between EGCG and peptide followed a Poisoned Growth Model. The peptides recovered by EGCG have much better solubility than acid precipitated peptides. This work establishes the feasibility of, and methodology for, recovering peptides from water solution using EGCG as an efficient carrier.

这项研究调查了使用表没食子儿茶素-3-没食子酸酯（EGCG）作为载体与大豆蛋白水解物水中的肽相互作用并从中沉淀出肽的可行性。将EGCG添加到大豆肽中，导致形成聚集物，这些聚集物在离心时沉淀。在EGCG浓度不同的酸性pH值为4.5和中性pH值为7.0的情况下，评估了多肽的回收效率。结果表明，肽回收率随着EGCG浓度的增加而增加。在pH 4.5和EGCG /肽比为0.40时，肽的最大回收率为57.17％。EGCG浓度的进一步增加不会导致肽回收率发生任何变化，这表明EGCG与肽之间的相互作用遵循中毒生长模型。通过EGCG回收的肽比酸沉淀的肽具有更好的溶解性。这项工作建立了使用EGCG作为有效载体从水溶液中回收肽的可行性和方法论。