Abstract The synthetic fiber manufacturing wastewater (FMW) contained large amounts of tetrahydrofuran (THF), 3-buten-1-ol (BDO) and 1,4-butanediol (BTO) and showed a high chemical oxygen demand (COD) of 7,992 ± 2,252 mg L−1. Ratio of the biological oxygen demand (BOD5) and COD of the FMW stream was analyzed as only 0.094, which reflected the low aerobically biodegradable property. For complementing existed aerobic biological treatment process, an external circulation sludge bed (ECSB) system consisting of two full-scale anaerobic granular sludge (AnGS) bioreactors was constructed and continuous operation for more than 25 months. Even though feed fluctuations occurred, robust performance of the system was exhibited with consistent high removals of COD (85.5 ± 4.4%), THF (79.8 ± 8.6%), BDO and BTO (both 100%). Fractionation analyses of AnGS at nine heights of the two bioreactors showed that the bigger size (>0.5 mm) dominated at the bottom (>92.1%). Morphology and elemental analyses of AnGS were helpful to see surface depositions in line with water analyses. Anaerobic biodegradations were also highlighted in a comprehensive analysis of carbon and nitrogen fractions, phosphates, sulfates and other minerals. Moreover, metagenomic analyses revealed Methanomicrobia and Methanosaeta in archaea and Exilispira in bacteria were the dominant genera in microbial community structure, which given more insights of anaerobic biodegradation in the two full-scale bioreactors.

中文翻译：

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用于低需氧生物降解合成纤维制造废水处理的全尺寸厌氧颗粒污泥生物反应器的长期性能和宏基因组分析

摘要 合成纤维制造废水 (FMW) 含有大量四氢呋喃 (THF)、3-丁烯-1-醇 (BDO) 和 1,4-丁二醇 (BTO)，化学需氧量 (COD) 高达 7,992 ± 2,252 毫克 L-1。经分析，FMW 流的生物需氧量 (BOD5) 和 COD 之比仅为 0.094，这反映了低需氧生物降解性。为了补充现有的好氧生物处理工艺，建造了由两个全尺寸厌氧颗粒污泥（AnGS）生物反应器组成的外循环污泥床（ECSB）系统，并连续运行了25个月以上。即使发生进料波动，系统仍表现出稳健的性能，COD (85.5 ± 4.4%)、THF (79.8 ± 8.6%)、BDO 和 BTO（均为 100%）的高去除率始终如一。AnGS 在两个生物反应器的九个高度的分级分析表明，较大的尺寸 (>0.5 mm) 在底部 (>92.1%) 占主导地位。AnGS 的形态学和元素分析有助于观察与水分析一致的表面沉积物。对碳和氮部分、磷酸盐、硫酸盐和其他矿物质的综合分析也强调了厌氧生物降解。此外，宏基因组分析显示古细菌中的甲烷微菌和甲烷菌和细菌中的 Exilispira 是微生物群落结构的优势属，这为两个全尺寸生物反应器中的厌氧生物降解提供了更多见解。AnGS 的形态学和元素分析有助于观察与水分析一致的表面沉积物。对碳和氮部分、磷酸盐、硫酸盐和其他矿物质的综合分析也强调了厌氧生物降解。此外，宏基因组分析显示古细菌中的甲烷微菌和甲烷菌和细菌中的 Exilispira 是微生物群落结构的优势属，这为两个全尺寸生物反应器中的厌氧生物降解提供了更多见解。AnGS 的形态学和元素分析有助于观察与水分析一致的表面沉积物。对碳和氮部分、磷酸盐、硫酸盐和其他矿物质的综合分析也强调了厌氧生物降解。此外，宏基因组分析显示古细菌中的甲烷微菌和甲烷菌和细菌中的 Exilispira 是微生物群落结构的优势属，这为两个全尺寸生物反应器中的厌氧生物降解提供了更多见解。