SNP markers linked to genes controlling Ca and Mn uptake were identified in the common bean seeds using DArT-based association mapping (AM). The Ca concentration in the seeds varied between 475 and 3,100 mg kg⁻¹ with an average of 1,280.9 mg kg⁻¹ and the Mn concentration ranged from 4.87 to 27.54 mg kg⁻¹ with a mean of 11.76 mg kg⁻¹. A total of 19,204 SNP markers were distributed across 11 chromosomes that correspond to the haploid genome number of the common bean. The highest value of ΔK was determined as K = 2, and 173 common bean genotypes were split into two main subclusters as POP1 (Mesoamerican) and POP2 (Andean). The results of the UPGMA dendrogram and PCA confirmed those of STRUCTURE analysis. MLM based on the Q + K model identified a large number of markers-trait associations. Of the 19,204 SNPs, five (on Pv2, 3, 8, 10 and 11) and four (on Pv2, 3, 8 and 11) SNPs were detected to be significantly related to the Ca content of the beans grown in Bornova and Menemen, respectively in 2015. In 2016, six SNPs (on Pv1–4, 8 and 10) were identified to be significantly associated with the Ca content of the seeds obtained from Bornova and six SNPs (on Pv1–4, 8 and 10) from Menemen. Eight (on Pv3, 5 and 11) and four (on Pv2, 5 and 11) SNPs had a significant association with Mn content in Bornova in 2015 and 2016, respectively. In Menemen, eight (on Pv3, 5, 8 and 11) and 11 (on Pv1, 2, 5, 10 and 11) SNPs had a significant correlation with Mn content in 2015 and 2016, respectively.

使用基于 DArT 的关联图谱 (AM) 在普通豆类种子中鉴定了与控制 Ca 和 Mn 吸收的基因相关的 SNP 标记。种子中的 Ca 浓度在 475 至 3,100 mg kg ^-1之间变化，平均为 1,280.9 mg kg ^-1，Mn 浓度范围为 4.87 至 27.54 mg kg ^-1，平均为 11.76 mg kg ^-1. 共有 19,204 个 SNP 标记分布在 11 条染色体上，对应于普通豆类的单倍体基因组编号。ΔK 的最高值被确定为 K = 2，173 个常见的豆类基因型被分成两个主要的亚群，即 POP1（中美洲）和 POP2（安第斯）。UPGMA 树状图和 PCA 的结果证实了 STRUCTURE 分析的结果。基于 Q+K 模型的 MLM 确定了大量标记-性状关联。在 19,204 个 SNP 中，检测到 5 个（在 Pv2、3、8、10 和 11 上）和 4 个（在 Pv2、3、8 和 11 上）SNP 与在 Bornova 和 Menemen 种植的豆类的 Ca 含量显着相关，分别在 2015 年。 2016 年，鉴定出六个 SNP（在 Pv1-4、8 和 10 上）与从 Bornova 和六个 SNP（在 Pv1-4、8 和 10）来自梅内门。8 个（在 Pv3、5 和 11 上）和四个（在 Pv2、5 和 11 上）SNP 分别与 2015 年和 2016 年博尔诺瓦的锰含量显着相关。在 Menemen 中，2015 年和 2016 年分别有 8 个（在 Pv3、5、8 和 11）和 11 个（在 Pv1、2、5、10 和 11）SNP 与 Mn 含量显着相关。