For an extensive period of time apical meristem (SAM) has been considered as a mysterious organ, due to its small, hidden and dynamic structure. Confocal imaging, combined with fluorescent reporters, enables researchers to unveil the mechanisms underlying cellular activities, such as gene expression, cell division, growth patterns and cell-cell communications. Recently, a series of protocols were developed for confocal imaging of inflorescence meristem (IM) and floral meristem (FM). However, the requirement of high configuration, such as the need of a water-dipping lens without coverslip and the specialized turrets associated with fixed-stage microscopes, impedes the wide adoption of these methods. We exploited an improved object slide and matching method aiming to decrease the configuration requirement. Following this protocol, various dry microscope lenses can be selected with flexibility for building 3D images of IM and FM.

在很长一段时间内，顶端分生组织（SAM）由于其小的，隐藏的和动态的结构而被认为是一个神秘的器官。共聚焦成像与荧光报告仪相结合，使研究人员能够揭示细胞活动的基础机制，例如基因表达，细胞分裂，生长方式和细胞间通讯。最近，开发了一系列协议用于花序分生组织（IM）和花分生组织（FM）的共聚焦成像。然而，对高配置的要求，例如需要不带盖玻片的浸水透镜以及与固定式显微镜相关的专用转塔，阻碍了这些方法的广泛采用。我们开发了一种改进的对象滑动和匹配方法，旨在降低配置要求。遵循此协议，