Recently, bacteriophage particles have started to be applied as a new biomaterial for developing sensing platforms. They can be used as both a recognition element or/and as building blocks, template/scaffold. In this paper, we studied a bacteriophage selected through phage-display technology. The chosen bacteriophage acted as a building block for creating a carbon nanofiber-based electrode and as a new receptor/binding element that recognizes C-reactive protein (CRP) – one of the markers of inflammatory processes in the human body. The binding efficiency of the selected phage towards CRP is two orders of magnitude higher than in the wild type. We demonstrate that the phage-based sensor is selective against other proteins. Finally, we show that layer-by-layer methods are suitable for deposition of negatively charged phages (wild or CRP-binding) with positively charged carbon nanofibers for electrode surface modification. A three-layered electrode was successfully used for molecular recognition of CRP, and the molecular interactions were studied using electrochemical, biological, and optical methods, including microscopic and spectroscopic analyses.

最近，噬菌体颗粒已开始被用作开发传感平台的新型生物材料。它们既可以用作识别元素，也可以用作模板/支架的构建基块。在本文中，我们研究了通过噬菌体展示技术选择的噬菌体。选定的噬菌体充当了创建基于碳纳米纤维的电极的基础，并且是识别C反应蛋白（CRP）的新受体/结合元件，C反应蛋白是人体炎症过程的标志之一。所选噬菌体对CRP的结合效率比野生型高两个数量级。我们证明基于噬菌体的传感器对其他蛋白质具有选择性。最后，我们显示逐层方法适用于带负电荷的噬菌体（野生或CRP结合）的沉积，带正电荷的碳纳米纤维用于电极表面修饰。三层电极已成功用于CRP的分子识别，并使用电化学，生物和光学方法（包括显微镜和光谱分析）研究了分子相互作用。