This study was to evaluate the effects of substituting fish meal (FM) with soy protein concentrate (SPC) on growth, non-specific immune function, antioxidant capacity and intestinal histology in juvenile golden crucian carp, × . Six isonitrogenous (40 % crude protein) and isolipidic (8 % crude lipid) diets were formulated with SPC to replace 0 % (FM control; SPC0), 20 % (SPC20), 40 % (SPC40), 60 % (SPC60), 80 % (SPC80) and 100 % (SPC100) of FM. Groups of golden crucian carp (initial weight 2.45 ± 0.01 g) fed each diets were cultured in triplicate 100-L tanks (50 fish per tank), and the fish were fed three times a day (07:00, 12:00 and 17:00) to apparent satiation for 8 weeks. Results showed that the growth performance and feed efficiency were significantly decreased when dietary FM was completely replaced by SPC compared with control group. However, no significant differences were found on feed intake (FI) and survival rate (SR) among all groups. Compared with the control group, offering SPC100 diets significantly reduced the crude lipid and crude protein contents of the whole-body and muscle. Similarly, the protease activities of the proximal intestine, mid intestine and distal intestine showed a similar trend, and the SPC100 group was significantly decreased than the SPC0 and SPC20 groups. Typical characteristics of distal intestinal damage are shown in the SPC100 group, including the atrophy of mucosal fold, widening of lamina propria and separation of epithelial mucosa from the lamina propria. The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in liver decreased with increasing dietary SPC levels. Additionally, replacing 80 % and 100 % of dietary FM with SPC will reduce the antioxidant capacity and non-specific immune function of golden crucian carp. These results indicate that under the reported conditions SPC may be included in the diet up to 393.4 g/kg as a substitute for FM, replacing about 80 % of FM in the diet of juvenile golden crucian carp.

中文翻译：

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大豆浓缩蛋白替代鱼粉对金鲫幼鱼生长性能、抗氧化能力及肠道组织学的影响


本研究旨在评价大豆浓缩蛋白（SPC）替代鱼粉（FM）对金鲫幼鱼生长、非特异性免疫功能、抗氧化能力和肠道组织学的影响。用 SPC 配制六种等氮（40% 粗蛋白）和异脂类（8% 粗脂）日粮，以代替 0%（FM 对照；SPC0）、20%（SPC20）、40%（SPC40）、60%（SPC60）、 FM 的 80 % (SPC80) 和 100 % (SPC100)。将各组饲料投喂的金鲫鱼（初重2.45±0.01g）在100L池中养殖三组（每池50条），每天投喂3次（07:00、12:00和17点）。 :00) 到明显饱腹感持续 8 周。结果表明，与对照组相比，当日粮中FM完全替代为SPC时，生长性能和饲料效率显着下降。然而，各组之间的采食量（FI）和成活率（SR）没有发现显着差异。与对照组相比，饲喂SPC100日粮显着降低了全身和肌肉的粗脂肪和粗蛋白含量。同样，近端肠、中肠和远端肠的蛋白酶活性表现出相似的趋势，且SPC100组较SPC0和SPC20组显着降低。 SPC100组显示远端肠道损伤的典型特征，包括粘膜皱襞萎缩、固有层增宽以及上皮粘膜与固有层分离。肝脏中天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）的活性随着膳食SPC水平的增加而降低。 另外，用SPC替代饲料中80%和100%的FM，会降低金鲫鱼的抗氧化能力和非特异性免疫功能。这些结果表明，在报道的条件下，SPC可以作为FM的替代品包含在饲料中高达393.4 g/kg，替代幼金鲫饲料中约80%的FM。