A strictly anaerobic bacterial strain designated as SKVG24 was isolated from subgingival dental plaque samples of patients suffering from periodontitis. Cells were stained Gram-positive, rod shaped with endospore. The strain showed negative reaction to catalase and oxidase enzymes, but positive for gelatinase activity. Optimal growth was observed at 37 °C temperature and 7.0 pH. The 16S rRNA gene sequence BLAST analysis assigned strain SKVG24 to the genus Paraclostridium as it displayed 99.93% identity with P. benzoelyticum JC272^T followed by P. bifermentans ATCC 638^T (99.79%). However, average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) of the whole genome sequence showed <97% and <70% identity, respectively, with type strains of all closely related species. The G + C content of the DNA was 28.7 mol%. Total lipids profile showed presence of glycolipids as major lipids. Pathogenic features like hemolysis, gelatin hydrolysis and production of volatile sulfur compounds exhibited by strain SKVG24^T were analogous to those observed in the established oral pathogenic strains. Further, whole genome sequence analysis confirmed the presence of genes encoding virulence factors and provided genomic insights on adaptation of the strain in oral environment. Based on the phenotypic and genetic differences with phylogenetic relatives, strain SKVG24^T is proposed to represent a new species of the genus Paraclostridium with potential pathogenic ability, for which the name Paraclostridium dentum sp. nov., is suggested. The proposed type strain is SKVG24^T (MTCC 12836^T; = JCM 32760^T).

从患有牙周炎的患者的龈下牙菌斑样品中分离出命名为SKVG24的严格厌氧细菌菌株。将细胞染色为革兰氏阳性，内生孢子形。该菌株对过氧化氢酶和氧化酶显示阴性反应，但对明胶酶活性显示阳性。在37°C温度和7.0 pH下观察到最佳生长。16S rRNA基因序列BLAST分析分配应变SKVG24属Paraclostridium因为它显示99.93％的同一性与P. benzoelyticum JC272 ^Ť随后P. bifermentans ATCC 638 ^Ť（99.79％）。然而，与所有密切相关物种的类型菌株相比，整个基因组序列的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）分别显示了<97％和<70％。DNA的G + C含量为28.7mol％。总脂质概况显示糖脂作为主要脂质存在。菌株SKVG24 ^T表现出的溶血，明胶水解和挥发性硫化合物生成等致病特征与已建立的口腔致病菌株相似。此外，全基因组序列分析证实了编码毒力因子的基因的存在，并提供了在口腔环境中适应菌株的基因组见解。根据与系统发生亲缘关系的表型和遗传差异，菌株SKVG24 ^T建议代表了属的一个新种Paraclostridium与潜在的致病能力，对于这名Paraclostridium dentum SP。建议。建议的类型应变为SKVG24 ^T（MTCC 12836 ^T ; = JCM 32760 ^T）。