The fig (Ficus carica L.), is known as a precious fruit tree for its nutrition and medicinal values, economic importance and for sustainable production in the semi-arid and arid areas. Expanding the cultivation of fig in new vulnerable areas and the breeding programs in fig need a reliable high-efficient system for in vitro morphogenesis to meet future demands. This study was carried to develop an efficient protocol for indirect regeneration of F. carica L. cultivars ‘Sabz’ and ‘Torsh’ using thin cell layer (TCL) technique. The genetic fidelity of the regenerated plants was also evaluated using flow cytometry technique and ISSR markers. Stem segments of 10 mm in diameter were taken from mature plants, then explants were transversally cut into layers of 0.5–0.8 mm thickness. Callus induction was successful using Murashige and Tucker (MT) medium supplemented with 9.08 μM TDZ plus 9.8 μM IBA (IM3 medium) which resulted in 50 ± 6.11% calli in ‘Sabz’ cultivar. Morphogenic calli were cut into small pieces and cultured on Murashige and Skoog (MS) medium for shoot development. Maximum shoot regeneration (45%) was observed in 17.68 μM BAP in combination with 4.54 μM TDZ and 1.07 μM NAA (RM2 medium), with an average of 6.9 shoots per explant. Flow cytometry and ISSR molecular marker analyses confirmed the stability of ploidy level and genetic identity of indirectly regenerated plants in both cultivars. The results of this study demonstrate that indirect regeneration of F. carica L. by the use of TCL system is a reliable and promising approach for future mass propagation programs as well as possible in vitro breeding objectives.

无花果（Ficus carica L.）因其营养和药用价值，经济重要性以及在半干旱和干旱地区的可持续生产而被称为珍贵的果树。在新的脆弱地区扩大无花果的种植和无花果的育种计划，需要一个可靠的高效体外形态发生系统，以满足未来的需求。进行该研究以开发用于F的间接再生的有效方案。卡里卡使用薄细胞层（TCL）技术的L.品种'Sabz'和'Torsh'。还使用流式细胞仪技术和ISSR标记评估了再生植物的遗传保真度。直径为10毫米的茎段取自成熟植物，然后将外植体横向切成0.5-0.8毫米厚的层。使用补充了9.08μMTDZ和9.8μMIBA（IM3培养基）的Murashige和Tucker（MT）培养基成功诱导了愈伤组织，这导致'Sabz'品种的愈伤组织达到50±6.11％。将形态发生的愈伤组织切成小块，并在Murashige和Skoog（MS）培养基上培养以进行芽发育。在17.68μMBAP与4.54μMTDZ和1.07μMNAA（RM2培养基）的组合中观察到最大的芽再生（45％），每个外植体平均有6.9个芽。流式细胞仪和ISSR分子标记分析证实了两个品种中间接再生植株的倍性水平和遗传同一性。这项研究的结果表明，间接再生˚F。通过使用TCL系统，carica L.是未来大规模繁殖计划以及可能的体外育种目标的可靠且有前途的方法。