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Cryopreservation and IVF in the time of Covid-19: what is the best good tissue practice (GTP)?
Journal of Assisted Reproduction and Genetics ( IF 3.2 ) Pub Date : 2020-08-03 , DOI: 10.1007/s10815-020-01904-5
Kimball O Pomeroy 1 , Mitchel C Schiewe 2
Affiliation  

Examine good tissue practices as relates to in vitro fertilization, biopsying, and vitrificationto compare current knowledge of ova, sperm, and embryos as vectors for disease transmission as it relates to our current knowledge regarding the SARS-CoV-2 virus.

Unknown risks relating to the SARS-CoV-2 virus and sperm, ova, and embryos necessitate a reexamining of how human IVF is performed. Over the last decade, improvements in cryosurvival and live birth outcomes have been associated with zona pellucida breaching procedures (e.g., blastocyst collapsing and biopsying). In turn, today embryos are generally no longer protected by an intact zona pellucida when vitrified and in cryostorage. Additionally, high security storage containers have proven to be resilient to potential cross-contamination and reliable for routine human sperm freezing and embryo vitrification.

Several options to current IVF practices are presented that can effectively mitigate the risks of cross-contamination and infection due to the current Covid-19 pandemic or other viral exposures. The question remains; is heightened security and change warranted where the risks of disease transmission likely remain negligible?



中文翻译:

Covid-19时期的冷冻保存和IVF:什么是最佳的良好组织惯例(GTP)?

检查与体外受精,活组织检查和玻璃化有关的良好组织规范,以比较卵,精子和胚胎作为疾病传播载体的当前知识,因为这与我们有关SARS-CoV-2病毒的当前知识有关。

与SARS-CoV-2病毒以及精子,卵子和胚胎有关的未知风险,需要重新检查人类IVF的执行方式。在过去的十年中,冷冻存活率和活产结局的改善与透明带透明带破损程序(例如囊胚塌陷和活检)有关。反过来,今天的胚胎通常在玻璃化和冷冻保存后不再受到完整透明带的保护。此外,事实证明,高安全性的储存容器可以抵抗潜在的交叉污染,并且对于常规的人类精子冷冻和胚胎玻璃化是可靠的。

提出了当前IVF做法的几种选择,可以有效减轻由于当前Covid-19大流行或其他病毒暴露而造成的交叉污染和感染的风险。问题仍然存在;在疾病传播的风险仍然可以忽略不计的情况下,是否应提高安全性和进行更改?

更新日期:2020-08-04
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