Haloarchaea have evolved to thrive in hypersaline environments. Haloferax volcanii is of particular interest due to its genetic tractability; however, few in vivo reporters exist for halophiles. Haloarchaeal proteins evolved characteristics that promote proper folding and function at high salt concentrations, but many mesophilic reporter proteins lack these characteristics. Mesophilic proteins that acquire salt-stabilizing mutations, however, can lead to proper function in haloarchaea. Using laboratory-directed evolution, we developed and demonstrated an in vivo luciferase that functions in the hypersaline cytosol of H. volcanii.

中文翻译：

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嗜盐古菌 Haloferax volcanii 的生物发光报告基因。

Haloarchaea 已经进化到在高盐环境中茁壮成长。Haloferax volcanii由于其遗传易处理性而特别受关注。然而，很少有嗜盐菌的体内报告基因。Haloarchaeal 蛋白质进化出在高盐浓度下促进正确折叠和功能的特征，但许多嗜温报告蛋白缺乏这些特征。然而，获得盐稳定突变的嗜温蛋白可以在盐古菌中发挥适当的作用。使用实验室定向进化，我们开发并展示了一种在H. volcanii的高盐度胞质溶胶中起作用的体内荧光素酶。