Here we describe creation of a model of intraocular retinoblastoma on 4 mature immunodeficient BALB/c nude mice of both sexes. A suspension of the primary retinoblastoma culture was injected into each of the 8 eyes of the test animals. The injections were performed under the control of an operating microscope using insulin syringes with a diameter of 29G transsclerally into the subretinal space in a volume of 0.3 ml at a rate of 10,000 cells in 25 μl physiological saline. The culture of Rb10 cells derived from a patient with retinoblastoma passed 12 passages at the time of the experiments. After 9-week follow-up, a clinical (ophthalmoscopy) and instrumental (ultrasound) study was performed with visualization of the tumor mass on the fundus. After enucleation, a morphological study was performed. The retinoblastoma tumor nodes were identified in two eyes. The proposed model can be used for further research and for testing new chemotherapeutic drugs and treatment regimens for retinoblastoma.

中文翻译：

---

眼内视网膜母细胞瘤体内模型的建立

在这里，我们描述了在 4 只成熟的免疫缺陷 BALB/c 裸鼠的两性眼内视网膜母细胞瘤模型的创建。将原代视网膜母细胞瘤培养物的悬浮液注射到测试动物的 8 只眼中。在手术显微镜的控制下使用直径为29G的胰岛素注射器以0.3ml的体积以10,000个细胞的速率在25μl生理盐水中经巩膜进入视网膜下空间进行注射。来自视网膜母细胞瘤患者的 Rb10 细胞培养物在实验时通过了 12 代。9 周随访后，进行临床（检眼镜）和仪器（超声）研究，观察眼底肿瘤块。摘除后，进行形态学研究。在两只眼睛中识别出视网膜母细胞瘤肿瘤节点。所提出的模型可用于进一步研究和测试新的化疗药物和视网膜母细胞瘤的治疗方案。