To understand the role of intestinal mucosal microbiota on mental stress-related diarrhoea, we collected the intestinal mucosa of mice treated with Folium senna extract gavage combined with restraint and tail pinch stress for 7 days; and intestinal mucosal microbiota characteristics were analyzed by 16S rRNA Pacbio SMRT gene full-length sequencing. The results showed that the diversity (i.e., alpha diversity including the Chao1, Simpson, ACE, and Shannon indices and beta diversity including the NMDS of weighted UniFrac distances) and composition of the microbial community in the intestinal mucosa of mice with diarrhoea and repeated stress changed significantly (P < 0.05). In the co-occurrence network, Staphylococcus sciuri and Escherichia fergusonii was identified as putative keystone species. Moreover, the characteristics of the intestinal microbial species was analyzed by LEfSe, Metastats, and group difference, and ten altered gut microbiota species can be used as characteristic microbes in the mice with diarrhoea and repeated stress: the abundances of Stigmatella aurantiaca, Candidatus arthromitus sp. SFB-mouse, Erythrobacter gaetbuli, Desulfitobacterium hafniense, Ochrobactrum pituitosum, and Candidatus arthromitus sp. SFB-mouse-NL in the model group were significantly lower than those in the control group (P < 0.05); whereas Microbacterium dextranolyticum, Klebsiella pneumoniae, Escherichia sp. BBDP27, and Streptococcus danieliae were enriched in the control group (P < 0.05). Collectively, mental stress-related diarrhoea increased the intestinal microbiota diversity. The species associated with mental stress-related diarrhoea including Microbacterium dextranolyticum, Klebsiella pneumoniae, Escherichia sp. BBDP27, and Streptococcus danieliae were significantly enriched; while the species which are beneficial to mental stress-related diarrhoea are Stigmatella aurantiaca, Candidatus arthromitus sp. SFB-mouse, Erythrobacter gaetbuli, Desulfitobacterium hafniense, Ochrobactrum pituitosum, and Candidatus arthromitus sp. SFB-mouse-NL for its significantly depleted.

为了解肠黏膜微生物群对精神压力相关腹泻的作用，我们收集了番泻叶提取物灌胃联合约束和捏尾应激治疗 7 天的小鼠肠黏膜；通过16S rRNA Pacbio SMRT基因全长测序分析肠道黏膜微生物群特征。结果表明，腹泻和反复应激小鼠肠黏膜微生物群落的多样性（即α多样性包括Chao1、Simpson、ACE和Shannon指数，β多样性包括加权UniFrac距离的NMDS）和微生物群落组成。变化显着（P  < 0.05）。在共生网络中，Staphylococcus sciuri和Escherichia fergusonii被确定为推定的关键物种。此外，通过LEfSe、Metastats和组差异分析肠道微生物种类的特征，10种改变的肠道微生物群可作为腹泻和反复应激小鼠的特征微生物：Stigmatella aurantiaca , Candidatus arthromitus sp . SFB鼠，赤gaetbuli，Desulfitobacterium hafniense，苍白杆菌pituitosum，并暂定arthromitus SP。模型组SFB-mouse-NL显着低于对照组（P  < 0.05）；然而右旋糖酐微杆菌、肺炎克雷伯菌、埃希氏菌。BBDP27和链球菌在对照组中富集（P  < 0.05）。总的来说，与精神压力相关的腹泻增加了肠道微生物群的多样性。与精神压力相关的腹泻相关的物种包括溶葡聚糖微杆菌、肺炎克雷伯菌、埃希氏菌属。BBDP27 和Streptococcus danieliae显着富集；而对精神紧张引起的腹泻有益的物种是橙花、关节假丝酵母sp. SFB鼠，赤gaetbuli，Desulfitobacterium hafniense，苍白杆菌pituitosum，并暂定arthromitus SP。SFB-mouse-NL为其显着耗尽。