In this study, a novel and stable gene transformation system was developed under control of Maize Proteinase Inhibitor (MPI) as an inducible promoter using the Mesoporous Silica Nanoparticles (MSNs). The functionalized MSNs with a proper particle size were synthesized and attached to a recombinant construct (pDNA) containing cryIAb gene under the control of MPI promoter (pPZP122:MPI:cryIAb:MSN [pDNA: MSN]) following transformation of tomato plants through injection of the pDNA: MSN complex into tomato red fruit at early ripening stage and then, putative transgenic seeds were collected. As an initial selection, gentamicin-resistant seedlings of T₁ (24.24%) and T₂ (61.37%) plants were identified. The transgene integration and expression were confirmed through the PCR, RT-PCR, and western blot approaches in the selected seedlings. PCR analysis showed that transformation frequency was equal to 10.71% in T₁ plants. Semi-quantitative RT-PCR analysis confirmed the transcript expression of cryIAb in all the T₁ and T₂ PCR-positive plants. Western blot analysis confirmed the existence of CryIAb protein in the leaves of T₂ putative transgenic plants. Accordingly, the results demonstrated that the transgene has more likely integrated into the tomato genome through homologous recombination. Bioassay was carried out for further assessment of the plant responses to Tuta absoluta resulting in an enhanced tolerance of the plant. In conclusion, the MSN-mediated stable transformation system under the MPI as an inducible promoter can be used as a suitable alternative for conventional genetic transformation methods due to its biodegradability, biocompatibility, cost and time-effectiveness, and positive effect on the plant defense against pathogens and pests.

在这项研究中，使用介孔二氧化硅纳米颗粒 (MSN) 在作为诱导型启动子的玉米蛋白酶抑制剂( MPI ) 的控制下开发了一种新型稳定的基因转化系统。合成具有适当粒径的功能化 MSN，并在通过注射番茄植株转化番茄植株后，在MPI启动子（pPZP122：MPI：cryIAb：MSN [pDNA：MSN]）的控制下，将含有cryIAb基因的重组构建体（pDNA）连接到pDNA：MSN 复合物在早熟阶段进入番茄红色果实，然后收集推定的转基因种子。作为初始选择，T _{1 的}庆大霉素抗性幼苗(24.24%) 和 T ₂ (61.37%) 植物被鉴定。通过 PCR、RT-PCR 和蛋白质印迹方法在选定的幼苗中证实了转基因整合和表达。PCR分析表明，T ₁植物的转化频率等于10.71% 。半定量RT-PCR 分析证实了cryIAb在所有T ₁和T ₂ PCR 阳性植物中的转录表达。Western印迹分析证实T ₂叶片中存在CryIAb蛋白假定的转基因植物。因此，结果表明转基因更有可能通过同源重组整合到番茄基因组中。进行生物测定以进一步评估植物对Tuta absoluta 的反应，从而提高植物的耐受性。总之，作为诱导型启动子的MPI下 MSN 介导的稳定转化系统由于其生物降解性、生物相容性、成本和时效性以及对植物防御抗逆转录病毒的积极作用，可用作常规遗传转化方法的合适替代品。病原体和害虫。