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Bioconversion of waste cooking oil for the production of poly-3-hydroxybutyrate using Bacillus cereus MPTDC
Indian Journal of Experimental Biology ( IF 0.7 ) Pub Date : 2020-08-03
Raveendran Sindhu, Athulya Manju, Pooja Mohan, Rajendran Omana Rajesh, Ranjna Sirohi, Aravind Madhavan, Parameswaran Binod

Used cooking oil is generated as a byproduct during frying process. It cannot be reused for cooking process due to health issues such as cancer and other digestive disorders. Alternative strategy is utilization of this waste cooking oil for production of poly-3-hydroxybutyrate (PHB) a biopolymer which can be used as a substitute for petroleum derived plastics or other value added products. In the present investigation, we used waste cooking oil as carbon source for PHB production by Bacillus cereus MPTDC. The optimum conditions of PHB production by Bacillus cereus MPTDC were waste cooking oil concentration of 2% (v/v), incubation time of 96 h, ammonium sulphate concentration of 7.5% and yeast extract concentration of 0.2%. Under optimized conditions the strain produced 3.777 g/L of PHB. The results indicate the potential of used cooking oil as carbon source for PHB production by Bacillus cereus MPTDC.

中文翻译:

使用蜡状芽孢杆菌MPTDC对废食用油进行生物转化以生产聚3-羟基丁酸酯

用过的食用油在油炸过程中会作为副产品生成。由于健康问题,例如癌症和其他消化系统疾病,它不能再用于烹饪过程。替代策略是利用这种废食用油生产生物聚合物聚-3-羟基丁酸酯(PHB),该聚合物可替代石油衍生的塑料或其他增值产品。在本次调查中,我们使用了废食用油作为蜡状芽孢杆菌MPTDC生产PHB的碳源。蜡状芽孢杆菌生产PHB的最佳条件MPTDC的废食用油浓度为2%(v / v),孵育时间为96小时,硫酸铵浓度为7.5%,酵母提取物浓度为0.2%。在优化条件下,菌株产生3.777 g / L的PHB。结果表明用过的食用油作为蜡状芽孢杆菌MPTDC生产PHB的碳源的潜力。
更新日期:2020-08-03
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