Functionalized gold nanoparticles are emerging as a promising nanocarrier for targeted delivery of therapeutic molecules. The present study describes synthesis, characterization and intracellular delivery of gold nanoparticles (AuNPs) functionalized with L1 protein of human papilloma virus peptide (HPV) derived cell-penetrating peptide (CPP) conjugated with R9. HPV-R9 cell penetrating peptide conjugated with AuNPs (fAuNPs) was characterized using UV-visible and Fourier Transform Infrared (FTIR) spectroscopy, transmission electron microscopy, dynamic light scattering and zeta potential. The cellular uptake of fAuNPs was studied in Vero cells using fluorescence microscopy and flow cytometry. The fAuNPs displayed reversible low intracellular aggregation, ~20% cytotoxicity but high efficiency and stability in Vero cells. The cytokines study by real-time PCR was performed in 4T1 cells of the IL-4, IL-12 and IFN-ᵞ, found to be upregulated in comparison to control. The functionalized gold nanoparticles cell penetrating peptide show good intracellular transport to thecellular destinations.Results of this study demonstrate that cell penetrating peptide can be used along with gold nanoparticles for therapeutics application, particularly cancer.

中文翻译：

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与基于人乳头瘤病毒（HPV-R9）的细胞穿透肽结合的金纳米颗粒在癌细胞中的增强的细胞内转运和细胞因子谱

功能化的金纳米颗粒正在作为一种有前途的纳米载体，用于靶向递送治疗性分子。本研究描述了金纳米颗粒（AuNPs）的合成，表征和细胞内递送，该纳米颗粒被人乳头瘤病毒肽（HPV）衍生的细胞穿透肽（CPP）的L1蛋白偶联了R9。使用紫外可见和傅里叶变换红外（FTIR）光谱，透射电子显微镜，动态光散射和Zeta电位对与AuNPs（fAuNPs）偶联的HPV-R9细胞穿透肽进行了表征。使用荧光显微镜和流式细胞术研究了Vero细胞中fAuNPs的细胞摄取。fAuNPs在Vero细胞中显示出可逆的低细胞内聚集，约20％的细胞毒性，但高效和稳定。通过实时PCR的细胞因子研究是在IL-4，IL-12和IFN-γ的4T1细胞中进行的，与对照相比，它们被上调了。功能化的金纳米颗粒细胞穿透肽显示出良好的细胞内转运至细胞目的地。这项研究结果表明，细胞穿透肽可以与金纳米颗粒一起用于治疗应用，尤其是癌症。