Cancer is the second most life threatening noncommunicable disease in humans that challenges the mankind with its multifactorial etiology. Even though various treatment strategies such as radiation therapy, chemotherapy, etc. are commonly used for this disease, researches are being focussed on developing plant-derived novel anticancer compounds that lack side effects. There were no previous reports on the anticancer potential of the ethnomedicinally important plant Anaphyllum wightii Schott. (Araceae). Hence, in the present study, we evaluated the in vitro cytotoxic effects of A. wightii on Dalton’s lymphoma ascites (DLA) tumor cell line. Interestingly, the acetone extract of rhizome showed much lower LC₅₀ value (14.27 μg/ mL) for the DLA cell line compared to that of normal rat spleen cells (1189.23μg/mL), revealing its significant anticancer potential. The molecular docking analysis showed β-sitosterol, present in the rhizome, as a promising lead molecule for the development of cytochrome P450 1 A1 inhibitors, which may provide potential anticancer agents.

中文翻译：

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Anaphyllum wightii Schott的体外抗癌潜力。对道尔顿淋巴瘤腹水细胞株及β-谷甾醇的分子对接研究

癌症是人类第二大威胁生命的非传染性疾病，其多因素病因向人类提出挑战。尽管该疾病通常使用多种治疗策略，例如放射疗法，化学疗法等，但研究仍集中在开发缺乏副作用的植物来源的新型抗癌化合物上。以前没有关于具有重要民族意义的植物Anaphyllum wightii Schott的抗癌潜力的报道。（天南星科）。因此，在本研究中，我们评估了A. wightii对道尔顿淋巴瘤腹水（DLA）肿瘤细胞系的体外细胞毒性作用。有趣的是，根茎的丙酮提取物显示出较低的LC与正常大鼠脾细胞（1189.23μg/ mL）相比，DLA细胞系的₅₀值（14.27μg/ mL）更显着。分子对接分析表明，根茎中存在的β-谷甾醇是开发细胞色素P450 1 A1抑制剂的有前途的先导分子，它可能提供潜在的抗癌药。