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Functional evaluation of exopolysaccharide from Pseudozyma sp. NII 08165 revealed the potential thickening and emulsifying applicability
Indian Journal of Experimental Biology ( IF 0.7 ) Pub Date : 2020-08-03
Kuttuvan Valappil Sajna, Rajeev Kumar Sukumaran, Lalitha Devi Gottumukkala, Sankar Sasidaran, Ashok Pandey

Exopolysaccharides (EPSs) have huge commercial potential as additives in food, personal care and pharmaceutical formulations. The physicochemical properties, in particularly rheological behaviour of an EPS, determine its potential application. In the present study, we investigated the structural, rheological, emulsifying, flocculating and water holding properties of Pseudozyma EPS in relation to its potential for industrial applications. Pseudozyma sp. NII 08165 was previously reported to produce an EPS of 1.7 MDa with high viscosity and pseudoplastic behaviour. Congo red assay indicated an ordered helical conformation of theEPS that could impart many desirable attributes. Comparative analysis of the EPS with respect to xanthan gum revealed that it had significant thickening efficiency and moderate suspending ability. Rheology of Pseudozyma EPS was stable up to 60°C and over the pH range 4.0-9.0. EPS exhibited superior flocculating activity and its emulsifying activity was as good as that of xanthan gum. The Pseudozyma EPS also demonstrated adequate water holding capacity. These results revealed that the Pseudozyma EPS could be used as a potential thickener, emulsifying agent or flocculating agent for diverse applications.

中文翻译:

假单胞菌胞外多糖的功能评估。NII 08165揭示了潜在的增稠和乳化适用性

胞外多糖(EPS)作为食品,个人护理和药物制剂中的添加剂具有巨大的商业潜力。EPS的物理化学性质(尤其是流变行为)决定了其潜在的应用。在本研究中,我们研究了假酶EPS的结构,流变,乳化,絮凝和持水性能及其在工业上的应用潜力。假酶sp。先前报道NII 08165具有高粘度和假塑性行为,可产生1.7 MDa的EPS。刚果红分析表明EPS有序的螺旋构象,可以赋予许多理想的属性。相对于黄原胶的EPS的比较分析表明,其具有显着的增稠效率和适度的悬浮能力。假酶EPS的变性在60°C和4.0-9.0的pH范围内都是稳定的。EPS表现出优异的絮凝活性,其乳化活性与黄原胶相同。该Pseudozyma EPS也表现出足够的保水能力。这些结果表明假酶 EPS可用作各种应用的潜在增稠剂,乳化剂或絮凝剂。
更新日期:2020-08-03
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