Human cytomegalovirus (HCMV) causes asymptomatic infections, but also causes congenital infections when women were infected with HCMV during pregnancy, and life-threatening diseases in immunocompromised patients. To better understand the mechanism of the neutralization activity against HCMV, the association of HCMV NT antibody titers was assessed with the antibody titers against each glycoprotein complex (gc) of HCMV. Sera collected from 78 healthy adult volunteers were used. HCMV Merlin strain and HCMV clinical isolate strain 1612 were used in the NT assay with the plaque reduction assay, in which both the MRC-5 fibroblasts cells and the RPE-1 epithelial cells were used. Glycoprotein complex of gB, gH/gL complexes (gH/gL/gO and gH/gL/UL128–131A [PC]) and gM/gN were selected as target glycoproteins. 293FT cells expressed with gB, gM/gN, gH/gL/gO, or PC, were prepared and used for the measurement of the antibody titers against each gc in an indirect immunofluorescence assay (IIFA). The correlation between the IIFA titers to each gc and the HCMV-NT titers was evaluated. There were no significant correlations between gB-specific IIFA titers and the HCMV-NT titers in epithelial cells or between gM/gN complex-specific IIFA titers and the HCMV-NT titers. On the other hand, there was a statistically significant positive correlation between the IIFA titers to gH/gL complexes and HCMV-NT titers. The data suggest that the gH/gL complexes might be the major target to induce NT activity against HCMV.

中文翻译：

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人巨细胞病毒（HCMV）中和抗体与针对HCMV糖蛋白复合物的抗体的关联。

人巨细胞病毒（HCMV）导致无症状感染，但孕妇在怀孕期间感染HCMV时也会引起先天性感染，并在免疫功能低下的患者中危及生命。为了更好地理解针对HCMV的中和活性的机制，将HCMV NT抗体效价与针对HCMV的每个糖蛋白复合物（gc）的抗体效价进行了评估。使用从78名健康成人志愿者那里收集的血清。HCMV Merlin菌株和HCMV临床分离株1612用于具有噬斑减少测定的NT测定中，其中同时使用了MRC-5成纤维细胞和RPE-1上皮细胞。选择了gB，gH / gL复合物（gH / gL / gO和gH / gL / UL128–131A [PC]）和gM / gN的糖蛋白复合物作为目标糖蛋白。293FT细胞以gB，gM / gN，gH / gL / gO，制备PC或PC或PC，用于间接免疫荧光测定（IIFA）中针对每种gc的抗体效价的测量。评估了每个gc的IIFA滴度和HCMV-NT滴度之间的相关性。上皮细胞中gB特异性IIFA滴度与HCMV-NT滴度之间，gM / gN复合体特异性IIFA滴度与HCMV-NT滴度之间无显着相关性。另一方面，IIFA滴度与gH / gL复合物和HCMV-NT滴度之间存在统计学上的显着正相关。数据表明，gH / gL复合物可能是诱导针对HCMV的NT活性的主要靶标。评估了每个gc的IIFA滴度和HCMV-NT滴度之间的相关性。上皮细胞中gB特异性IIFA滴度与HCMV-NT滴度之间或gM / gN复合体特异性IIFA滴度与HCMV-NT滴度之间无显着相关性。另一方面，IIFA滴度与gH / gL复合物和HCMV-NT滴度之间存在统计学上的显着正相关。数据表明，gH / gL复合物可能是诱导针对HCMV的NT活性的主要靶标。评估了每个gc的IIFA滴度和HCMV-NT滴度之间的相关性。上皮细胞中gB特异性IIFA滴度与HCMV-NT滴度之间或gM / gN复合体特异性IIFA滴度与HCMV-NT滴度之间无显着相关性。另一方面，IIFA滴度与gH / gL复合物和HCMV-NT滴度之间存在统计学上的显着正相关。数据表明，gH / gL复合物可能是诱导针对HCMV的NT活性的主要靶标。IIFA滴度与gH / gL复合物和HCMV-NT滴度之间存在统计学上的显着正相关。数据表明，gH / gL复合物可能是诱导针对HCMV的NT活性的主要靶标。IIFA滴度与gH / gL复合物和HCMV-NT滴度之间存在统计学上的显着正相关。数据表明，gH / gL复合物可能是诱导针对HCMV的NT活性的主要靶标。