Pomacea canaliculata, known as an invasive freshwater snail, is also called a golden apple snail; its survival and expansion are greatly affected by temperature. In this study, high-throughput sequencing (RNA-seq) was used to perform comparative transcriptome analysis on the muscular tissue (G_M) of snails in Guangdong and Hunan. Differential gene screening was performed with FDR <0.05 and |log2FoldChange| >1 as the threshold, and a total of 1,368 differential genes were obtained (671 genes showed upregulation in snails from Guangdong, and 697 genes displayed upregulation in snails from Hunan). Fifteen genes were identified as candidate genes for the cold hardiness of Pomacea canaliculata. Among them, three genes were involved in energy metabolism (glycogen synthase, 1; DGK, 1; G6PD, 1); seven genes were involved in homeostasis regulation (HSP70, 2; BIP, 1; GPX, 1; GSTO 1, G6PD, 1; caspase-9, 1); two genes were involved in amino acid metabolism (glutamine synthetase, 1; PDK, 1); and four genes were involved in membrane metabolism (inositol-3-phosphate synthase, 1; Na⁺/K⁺-ATPase, 1; calcium-binding protein, 2). This study presents the molecular mechanisms for the cold hardiness of Pomacea canaliculata, which could provide a scientific basis for the forecast and prevention of harm from Pomacea canaliculata.

中文翻译：

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分析广东省和湖南省小菜蛾抗寒性差异的转录组学比较方法。

Pomacea canaliculata，被称为侵入性淡水蜗牛，也被称为金苹果蜗牛。其生存和扩展受到温度的极大影响。在这项研究中，高通量测序（RNA-seq）用于对广东和湖南蜗牛的肌肉组织（G_M）进行比较转录组分析。差异基因筛查采用FDR <0.05和| log2FoldChange |进行。> 1作为阈值，总共获得了1,368个差异基因（广东的蜗牛中有671个基因上调，湖南的蜗牛中有697个基因上调）。鉴定出十五个基因作为加拿大冰果耐寒性的候选基因。其中，三个基因参与能量代谢（糖原合酶，1； DGK，1； G6PD，1）；糖原合成酶。七个基因参与稳态调节（HSP70，2; BIP，1; GPX，1; GSTO 1，G6PD，1; caspase-9，1）; 氨基酸代谢涉及两个基因（谷氨酰胺合成酶，1； PDK，1）；膜代谢涉及四个基因（肌醇-3-磷酸合酶1； Na ⁺ / K ⁺ -ATPase 1；钙结合蛋白2）。本研究提出了油菜（Pomacea canaliculata）抗寒性的分子机制，可以为油菜（Pomacea canaliculata）危害的预测和预防提供科学依据。