The Mediterranean fruit fly Ceratitis capitata is a globally invasive pest, often controlled with the sterile insect technique (SIT). For the SIT, mass-rearing of the target insect followed by irradiation are imperatives. Sterile males are often less able to inhibit female remating and transfer less number of sperm, and even irradiation could affect male reproductive organs, with consequences for their ability to inhibit female remating. On the other hand, male age could affect their ability to modulate female response after mating. Here, we evaluated the quality of the genetic sexing strain Vienna-8-tsl mass-reared in Bioplanta San Juan, Argentina, under laboratory conditions, with regard to: (i) the ability of sterile males irradiated at 100 or 140 Gy to inhibit female remating, in the same day and at 24 h of first copulation; (ii) the ability of 3, 4 or 5 day-old sterile males to inhibit female remating at 24 h of first copulation, and (iii) the effect of a reduction in irradiation doses on the number of sperm stored by females and reproductive organ size in virgin males. Sterile males were better able than wild males to inhibit female remating in the same day of first copulation and as able as wild males 1 day after first copulation. Male age did not affect their ability to inhibit female receptivity. Number of sperm stored by females, testes size and ectodermal accessory glands size were not affected by male identity, while sterile 100 Gy males had larger mesodermal accessory glands than control lab males. A reduction in irradiation dose does not impact any variable measured, except for percentage of sperm-depleted females: females mated with sterile 100 Gy males had lower probabilities to store sperm. The results showed here are very encouraging for tsl Vienna 8 strain reared in Argentina and are discussed in comparison with previous studies in C. capitata female remating with dissimilar results.

中文翻译：

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辐照剂量不影响男性生殖器官大小、精子储存和女性再交配倾向

地中海果蝇头孢菌素是一种全球入侵性害虫，通常通过昆虫不育技术 (SIT) 进行控制。对于 SIT，目标昆虫的大规模饲养和辐照是当务之急。不育雄性通常不太能够抑制雌性交配和转移较少数量的精子，甚至辐射也会影响雄性生殖器官，从而影响它们抑制雌性交配的能力。另一方面，男性年龄可能会影响他们在交配后调节女性反应的能力。在这里，我们在实验室条件下评估了在阿根廷圣胡安 Bioplanta San Juan 大规模饲养的遗传性别鉴定菌株 Vienna-8-tsl 的质量：(i) 不育雄性在 100 或 140 Gy 辐射下抑制的能力雌性交配，在同一天和首次交配后 24 小时；(ii) 3 的能力，4 或 5 天大的不育雄性在第一次交配后 24 小时抑制雌性重新交配，以及 (iii) 减少辐照剂量对雌性储存的精子数量和处女雄性生殖器官大小的影响。在第一次交配的同一天，不育雄性比野生雄性更能抑制雌性再交配，在第一次交配后 1 天与野生雄性一样。男性年龄不影响他们抑制女性接受能力的能力。女性储存的精子数量、睾丸大小和外胚层附属腺体大小不受男性身份的影响，而不育 100 Gy 男性的中胚层附属腺体比对照实验室男性大。辐射剂量的减少不会影响任何测量的变量，除了精子耗尽的女性百分比：与不育的 100 Gy 雄性交配的雌性储存精子的可能性较低。这里显示的结果非常令人鼓舞tslVienna 8 菌株在阿根廷饲养，并与之前在阿根廷的研究进行了比较讨论C.capita结果不同的雌性交配。