Label-free, non-contact imaging with mechanical contrast and optical sectioning is a substantial challenge in microscopy. Spontaneous Brillouin scattering microscopy meets this challenge, but encounters a trade-off between acquisition speed and the specificity for biomechanical constituents with overlapping Brillouin bands. Stimulated Brillouin scattering microscopy overcomes this trade-off and enables the cross-sectional imaging of live Caenorhabditis elegans at the organ and subcellular levels, with both elasticity and viscosity contrasts at high specificity and with practical recording times.

中文翻译：

---

高灵敏度和高特异性生物力学成像的刺激布里渊散射显微镜。

具有机械对比度和光学切片的无标签，非接触式成像是显微镜领域的重大挑战。自发的布里渊散射显微镜可以解决这一难题，但是在获取速度和布里渊带重叠的生物力学成分的特异性之间会进行权衡。刺激的布里渊散射显微镜克服了这一折衷，并使活的秀丽隐杆线虫在器官和亚细胞水平上进行截面成像，在高特异性和实际记录时间下具有弹性和粘度对比。