Abstract

In the current study, a novel mixed-ligand coordination polymer, {[Co(L)(dpe)]·(ClO₄)} (1) (H₂LCl = 3-carboxy-1-(3-carboxybenzyl)pyridin-1-ium, dpe = (E)-1,2-di(pyridin-4-yl)ethene), was synthesized under hydrothermal conditions by a zwitterionic organic ligand and fully characterized by the single crystal X-ray diffraction, powder X-ray diffraction, elemental analysis along with the FT-IR. Furthermore, the treatment activity of the compound on the ulcerative colitis was evaluated and the related mechanism was explored. First, the level of the tumor necrosis factor (TNF)-α and superoxide dismutase (SOD) released by the colon tissue into the plasma was measured with enzyme-linked immunosorbent assay (ELISA) detection kit. In addition to this, the activation level of the JAK2/STAT3 signaling pathway in the colonic epithelial cells was also determined with western blot. With the help of molecular docking simulation, the potential interacting gestures between the complex and the protein have been revealed.

抽象的

在当前的研究中，一种新型的混合配体配位聚合物{[Co（L）（dpe）]·（ClO ₄）}（1）（H ₂LCI = 3-羧基-1-（3-羧基苄基）吡啶-1-鎓，dpe =（E）-1,2-二（吡啶-4-基）乙烯），是通过两性离子有机配体在水热条件下合成的并具有单晶X射线衍射，粉末X射线衍射，元素分析以及FT-IR的特征。此外，还对该化合物对溃疡性结肠炎的治疗活性进行了评估，并探讨了其相关机制。首先，使用酶联免疫吸附测定（ELISA）检测试剂盒测量了结肠组织释放到血浆中的肿瘤坏死因子（TNF）-α和超氧化物歧化酶（SOD）的水平。除此之外，还通过蛋白质印迹法测定了结肠上皮细胞中JAK2 / STAT3信号传导途径的激活水平。借助分子对接模拟，