ABSTRACT CAPRICE (CPC), an R3-type MYB transcription factor, is known to promote root hair differentiation in the root epidermis of Arabidopsis. CPC moves from non-hair cells to adjacent hair-forming cells. In contrast, we have previously shown that there is no movement of the CPC homologs, ENHANCER OF TRY AND CPC 1, 2, and 3 (ETC1, 2, and 3), and TRYPTICHON (TRY) between root epidermal cells. We also identified a tomato homolog of CPC, named Solanum lycopersicumTRYPTICHON (SlTRY).SlTRY-introduced transgenic Arabidopsis produced many root hairs, like CPC-introduced transgenic Arabidopsis. To clarify the cell-to-cell movement ability of the SlTRY protein, in this study, we observed the distribution of GFP fluorescence in CPCp:SlTRY:GFP transgenic Arabidopsis. Unexpectedly, SlTRY moved from non-hair cells to adjacent root hair cells, like CPC, in Arabidopsis root epidermis. SlTRY does not have the cell-to-cell movement sequence (S1) defined in CPC, and the mechanism of movement is still unknown. Further investigation is necessary to elucidate the mechanism of cell-to-cell movement of SlTRY. Our results will help in the further unraveling of the functions of these MYB transcription factors in determining cell fate.

中文翻译：

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由 SlTRY 编码的番茄 (Solanum lycopersicum) R3 MYB 蛋白在拟南芥根中的定位

摘要 CAPRICE (CPC) 是一种 R3 型 MYB 转录因子，已知可促进拟南芥根表皮中的根毛分化。CPC 从非毛细胞移动到相邻的毛发形成细胞。相比之下，我们之前已经表明，根表皮细胞之间没有 CPC 同系物、ENHANCER OF TRY AND CPC 1、2 和 3（ETC1、2 和 3）和 TRYPTICHON (TRY) 的移动。我们还鉴定了 CPC 的番茄同系物，命名为 Solanum lycopersicumTRYPTICHON (SlTRY)。SlTRY 引入的转基因拟南芥产生了许多根毛，如 CPC 引入的转基因拟南芥。为了阐明 SlTRY 蛋白的细胞间运动能力，在本研究中，我们观察了 GFP 荧光在 CPCp:SlTRY:GFP 转基因拟南芥中的分布。出乎意料的是，SlTRY 从非毛细胞移动到相邻的根毛细胞，如 CPC，在拟南芥根表皮中。SlTRY没有CPC中定义的cell-to-cell运动序列（S1），运动机制尚不清楚。需要进一步研究以阐明 SlTRY 细胞间运动的机制。我们的结果将有助于进一步阐明这些 MYB 转录因子在决定细胞命运方面的功能。