Abstract

Some populations of the East Asian minnow Pungtungia herzi Herzenstein, 1892, which are naturally distributed in western Japan, have declined and are considered endangered. However, P. herzi has become a domestic invasive species in eastern Japan. Thus, knowledge of genetic features and phylogenetic relationships of P. herzi is important for conservation of this species and understanding its impact on ecosystems. We analyzed the complete mitochondrial genome using next generation sequencing of the East Asian minnow P. herzi from Yodo River, Osaka Prefecture, Japan. The mitochondrial genome of P. herzi consists of a circular molecule of 16,599 bp that includes 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and one control region. The heavy (H)-strand was predicted to have 12 PCGs, 14 tRNA, and two rRNA genes, while the light (L)-strand was predicted to contain one PCGs and eight tRNA genes. The average AT content was 57.68%. The genes ATP8 and ATP6, ATP6 and COIII, ND4L and ND4, and ND5 and ND6 shared seven, one, seven, and four nucleotides, respectively. The initiation codons ATG and GTG were found in 12 and one genes, respectively. The termination codons TAA, TAG, incomplete TA–, and single T–– were observed in nine, one, one, and two genes, respectively. All the tRNA genes possessed a cloverleaf secondary structure. The phylogenetic relationships inferred using 13 PCGs (based on the maximum likelihood) were consistent with previous studies that predicted interrelationships of Cypriniformes.

摘要

在日本西部自然分布的1892年东亚小now鱼Pungtungia herzi Herzenstein的种群数量下降，并被视为濒危物种。然而，P.herzi已成为日本东部的一种家庭入侵物种。因此，了解赫氏疟原虫的遗传特征和系统发育关系对于保护该物种和了解其对生态系统的影响非常重要。我们使用来自日本大阪府淀川的东亚min鱼P.herzi的下一代测序技术分析了完整的线粒体基因组。赫氏疟原虫线粒体基因组由16599 bp的环状分子组成，包括13个蛋白质编码基因（PCG），22个tRNA基因，两个rRNA基因和一个控制区。重（H）链预计具有12个PCG，14个tRNA和2个rRNA基因，而轻（L）链预计包含1个PCG和8个tRNA基因。平均AT含量为57.68％。基因ATP8和ATP6，ATP6和COIII，ND4L和ND4，以及ND5和ND6分别共享七个，一个，七个和四个核苷酸。在12个和一个基因中分别发现了起始密码子ATG和GTG。在9个，1个，1个和2个基因中分别观察到终止密码子TAA，TAG，不完全TA–和单个T–。所有的tRNA基因都具有苜蓿叶形二级结构。