ABSTRACT

Human papillomavirus (HPV) is believed to cause cervical cancer. Thousands of women develop cancer and other diseases caused by HPV each year. HPV 16 and 18 types are found in approximately 70% of cervical cancers. Micronuclei are small chromosomal fragments that are considered indicators of DNA damage. AgNOR positive dots are useful for assessing proliferation. We investigated the relation between HPV-DNA, micronuclei and AgNOR in smear samples. Three groups were defined: HPV negative, 16/18 positive and other high-risk groups (31, 33, 35, 39, 45, 51, 52, 56, 58, 66 and 68) (HR). After typing, micronuclei were identified by Papanicolaou staining and AgNOR regions were detected by silver staining. Serum reactive protein (CRP) also was measured. We found that the average age of HPV negative patients was significantly greater than for the HPV positive groups. We also found that CRP levels were significantly higher in the HPV 16/18 positive group than HPV negative and other HPV group. We found that the number of micronuclei in the HPV 16/18 group was significantly greater than for the HPV negative group. Also, we found that AgNOR staining for the HPV 16/18 group was significantly greater than for the HPV negative group. We found that CRP level, cell proliferation and genome instability were increased in HPV positive patients. The AgNOR and micronucleus tests were useful for evaluating cell proliferation and DNA damage.

摘要

人乳头瘤病毒 (HPV) 被认为会导致宫颈癌。每年有数千名妇女患上由 HPV 引起的癌症和其他疾病。在大约 70% 的宫颈癌中发现了 HPV 16 和 18 型。微核是小的染色体片段，被认为是 DNA 损伤的指标。AgNOR 阳性点可用于评估增殖。我们研究了涂片样本中 HPV-DNA、微核和 AgNOR 之间的关系。定义了三组：HPV 阴性、16/18 阳性和其他高危组（31、33、35、39、45、51、52、56、58、66 和 68）（HR）。分型后，巴氏染色鉴定微核，银染检测AgNOR区域。还测量了血清反应蛋白（CRP）。我们发现 HPV 阴性患者的平均年龄显着大于 HPV 阳性组。我们还发现 HPV 16/18 阳性组的 CRP 水平显着高于 HPV 阴性和其他 HPV 组。我们发现 HPV 16/18 组的微核数量显着多于 HPV 阴性组。此外，我们发现 HPV 16/18 组的 AgNOR 染色显着高于 HPV 阴性组。我们发现 HPV 阳性患者的 CRP 水平、细胞增殖和基因组不稳定性增加。AgNOR 和微核测试可用于评估细胞增殖和 DNA 损伤。我们发现 HPV 16/18 组的微核数量显着多于 HPV 阴性组。此外，我们发现 HPV 16/18 组的 AgNOR 染色显着高于 HPV 阴性组。我们发现 HPV 阳性患者的 CRP 水平、细胞增殖和基因组不稳定性增加。AgNOR 和微核测试可用于评估细胞增殖和 DNA 损伤。我们发现 HPV 16/18 组的微核数量显着多于 HPV 阴性组。此外，我们发现 HPV 16/18 组的 AgNOR 染色显着高于 HPV 阴性组。我们发现 HPV 阳性患者的 CRP 水平、细胞增殖和基因组不稳定性增加。AgNOR 和微核测试可用于评估细胞增殖和 DNA 损伤。