Background: Bovine mastitis is a growing health problem, affecting both welfare of dairy cattle and milk production. It often leads to chronic infections, disturbing the quality of milk and resulting in cow death. Thus, it has a great economic impact for breeders. Methods: In this study, we evaluated the protective effect of hydroxytyrosol—a natural molecule which is the major constituent of many phyto-complexes—in an in vitro model of mastitis induced by LPS (1μg/mL). Results: Our results showed that hydroxytyrosol (10 and 25 μM) was able to prevent the oxidative stress induced by LPS (intracellular ROS, GSH and NOX-1) and the consequently inflammatory response (TNF-α, IL-1β and IL-6). The protective effect of hydroxytyrosol is also related to the enhancement of endogenous antioxidant systems (Nrf2, HO-1, NQO-1 and Txnrd1). Moreover, hydroxytyrosol showed an important protective effect on cell functionality (α-casein S1, α-casein S2 and β-casein). Conclusions: Taken together, our results showed a significant protective effect of hydroxytyrosol on oxidative stress and inflammatory response in MAC-T cells. Thus, we indicated a possible important therapeutic role for hydroxytyrosol in the prevention or management of bovine mastitis.

中文翻译：

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羟基酪醇对脂多糖诱导的牛乳腺上皮细胞炎症和氧化应激的影响：牛乳腺炎的天然治疗工具。

背景：牛乳腺炎是一个日益严重的健康问题，它影响奶牛的福利和牛奶生产。它通常会导致慢性感染，干扰牛奶质量并导致奶牛死亡。因此，它对育种者具有巨大的经济影响。方法：在这项研究中，我们评估了羟基酪醇（一种天然分子，是许多植物复合物的主要成分）在LPS（1μg/ mL）诱导的乳腺炎的体外模型中的保护作用。结果：我们的结果表明，羟基酪醇（10和25μM）能够预防LPS（细胞内ROS，GSH和NOX-1）诱导的氧化应激，从而防止炎症反应（TNF-α，IL-1β和IL-6） ）。羟基酪醇的保护作用还与增强内源性抗氧化剂系统（Nrf2，HO-1，NQO-1和Txnrd1）有关。此外，羟基酪醇对细胞功能（α-酪蛋白S1，α-酪蛋白S2和β-酪蛋白）具有重要的保护作用。结论：综上所述，我们的结果表明羟基酪醇对MAC-T细胞的氧化应激和炎症反应具有显着的保护作用。因此，我们表明羟基酪醇在预防或管理牛乳腺炎中可能具有重要的治疗作用。