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Effect of Nanoconfinement of Polyphenolic Extract from Grape Pomace into Functionalized Mesoporous Silica on Its Biocompatibility and Radical Scavenging Activity.
Antioxidants ( IF 6.0 ) Pub Date : 2020-08-03 , DOI: 10.3390/antiox9080696
Ana-Maria Brezoiu 1 , Laura Bajenaru 1 , Daniela Berger 1 , Raul-Augustin Mitran 2 , Mihaela Deaconu 1 , Daniel Lincu 1, 2 , Anicuta Stoica Guzun 3 , Cristian Matei 1 , Mihaela Georgeta Moisescu 4, 5 , Ticuta Negreanu-Pirjol 6
Affiliation  

The aim of this paper is to assess the properties of Mamaia (MM) grape pomace polyphenolic extract loaded onto pristine and functionalized MCM-41 mesoporous silica as potential ingredients for nutraceuticals or cosmetics. The chemical profile of hydroalcoholic polyphenolic extracts, prepared either by conventional extraction or microwave-assisted method, was analyzed by reverse-phase high-performance liquid chromatography with photodiode array detector (HPLC-PDA) analysis, while their radical scavenger activity (RSA) was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assays. The extract-loaded materials were characterized by Fourier transform infrared (FTIR) spectroscopy, N2 adsorption-desorption isotherms, thermogravimetric analysis, as well as RSA (DPPH and ABTS assays). The polyphenols release profiles from pristine and functionalized (with mercaptopropyl, propyl sulfonic acid, cyanoethyl and propionic acid moieties) MCM-41-type supports were determined in phosphate buffer solution (PBS) pH 5.7. For selected materials containing embedded phytochemicals, cellular viability, and oxidative stress level on immortalized mouse embryonic fibroblast cell line (NIH3T3) were evaluated. A more acidic functional groups linked on silica pore walls determined a higher amount of phytochemicals released in PBS. The extract-loaded materials showed a good cytocompatibility on tested concentrations. The embedded extract preserved better the RSA over time than the free extract. The polyphenols-loaded MCM-41-type silica materials, especially material, demonstrated a good in vitro antioxidant effect on NIH3T3 cells, being potential candidates for nutraceutical or cosmetic formulations. View Full-Text

中文翻译:


功能化介孔二氧化硅纳米限制葡萄渣多酚提取物对其生物相容性和自由基清除活性的影响。



本文的目的是评估负载在原始和功能化 MCM-41 介孔二氧化硅上的 Mamaia (MM) 葡萄渣多酚提取物作为营养保健品或化妆品的潜在成分的特性。采用反相高效液相色谱-光电二极管阵列检测器 (HPLC-PDA) 分析了通过常规提取或微波辅助方法制备的水醇多酚提取物的化学特征,同时测定了其自由基清除剂活性 (RSA)。使用 DPPH(2,2-二苯基-1-三硝基苯肼自由基)和 ABTS(2,2'-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)测定法进行评估。提取物负载材料通过傅里叶变换红外进行表征( FTIR)光谱、N 2吸附-解吸等温线、热重分析以及 RSA(DPPH 和 ABTS 测定)从原始和功能化(带有巯基丙基、丙基磺酸、氰乙基和丙酸部分)MCM-41 中的多酚释放曲线。在pH 5.7的磷酸盐缓冲溶液(PBS)中测定了类型支持物。对于含有嵌入植物化学物质的选定材料,评估了永生化小鼠胚胎成纤维细胞系(NIH3T3)的细胞活力和氧化应激水平。二氧化硅孔壁上连接的酸性官能团越多,PBS 中释放的植物化学物质的量就越多。负载提取物的材料在测试浓度下表现出良好的细胞相容性。随着时间的推移,嵌入的提取物比免费提取物更好地保存了 RSA。负载多酚的MCM-41型二氧化硅材料,特别是材料,对NIH3T3细胞表现出良好的体外抗氧化作用,是营养保健品或化妆品配方的潜在候选者。查看全文
更新日期:2020-08-03
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