Mycobacterium chimaera is an opportunistic slowly growing non-tuberculous mycobacteriumof increasing importance due to the outbreak of cases associated with contaminated 3T heater-cooler device (HCD) extracorporeal membrane oxygenator (ECMO). The aim of this study was to evaluate the effect of pre-treating a surface with a Methylobacterium sp. CECT 7180 extract to inhibit the M. chimaera ECMO biofilm as well as of the treatment after different dehydration times. Surface adherence, biofilm formation and treatment effect were evaluated by estimating colony-forming units (CFU) per square centimeter and characterizing the amount of covered surface area, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. We found that exposing a surface to the Methylobacterium sp. CECT 7180 extract inhibited M. chimaera ECMO biofilm development. This effect could be result of the effect of Methylobacterium proteins, such as DNaK, trigger factor, and xanthine oxidase. In conclusion, exposing a surface to the Methylobacteriumsp. extract inhibits M. chimaera ECMO biofilm development. Furthermore, this extract could be used as a pre-treatment prior to disinfection protocols for equipment contaminated with mycobacteria after dehydration for at least 96 h.

中文翻译：

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甲基杆菌属的接触效应。从 3T 加热器-冷却器系统分离的嵌合分枝杆菌菌株生物膜提取物。

嵌合分枝杆菌是一种机会性缓慢生长的非结核分枝杆菌，由于与受污染的 3T 加热器冷却装置 (HCD) 体外膜氧合器 (ECMO) 相关的病例爆发，其重要性日益增加。本研究的目的是评估用甲基杆菌预处理表面的效果。CECT 7180 提取物对嵌合微球菌ECMO 生物膜的抑制作用以及不同脱水时间后的处理效果。通过估计每平方厘米的菌落形成单位 (CFU) 并使用共焦激光扫描显微镜和表征不同时间的覆盖表面积、厚度、细胞活力和固有自发荧光的存在来评估表面粘附、生物膜形成和治疗效果。图像分析。我们发现将表面暴露于甲基杆菌属。CECT 7180 提取物抑制M. chimaera ECMO 生物膜的发育。这种效应可能是甲基杆菌蛋白（例如 DNAK、触发因子和黄嘌呤氧化酶）作用的结果。总之，将表面暴露于甲基杆菌属。提取物抑制M. chimaera ECMO 生物膜的发育。此外，该提取物可用作脱水至少 96 小时后受分枝杆菌污染的设备消毒方案之前的预处理。