Phthalates, including di‐(2‐ethylhexyl)phthalate (DEHP), are common industrial chemicals in the environment. Recent evidence indicates that DEHP and its active metabolite mono‐(2‐ethylhexyl)phthalate (MEHP) negatively modulate reproductive functions and induce reactive oxygen species. Ascorbic acid (AA) is a dietary requirement for primates, and it acts as a potent free radical scavenger to protect tissues against oxidative stress. In this study, to investigate the toxic effects of MEHP on the follicle development and the beneficial role of AA, neonatal mouse ovaries were treated with different concentrations of MEHP with or without AA for 6 days. Then, the follicle constitution and oxidative status were compared in different groups. Results showed MEHP accelerated primordial follicle recruitment by increasing the percentage of primary and secondary follicles and decreasing the percentage of primordial follicles in the ovaries. Moreover, MEHP‐induced ovarian oxidative stress by significantly increasing malondialdehyde (MDA) concentration and the expression of GSS and SOD1. When ovaries were co‐administrated with MEHP and AA, follicle constitution was normalized, and the oxidative status was significantly decreased. These results suggested that AA ameliorated MEHP‐induced ovarian oxidative stress and follicular dysregulation, which attested the clinical significance of AA for ovary protection in the case of MEHP exposure.

中文翻译：

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抗坏血酸通过减少卵巢氧化应激改善新生小鼠卵巢中由邻苯二甲酸单-（2-乙基己基）酯诱导的失调的卵泡发生。

邻苯二甲酸盐，包括邻苯二甲酸二(2-乙基己基)酯 (DEHP)，是环境中常见的工业化学品。最近的证据表明，DEHP 及其活性代谢物邻苯二甲酸单(2-乙基己基)酯 (MEHP) 会负面调节生殖功能并诱导活性氧。抗坏血酸 (AA) 是灵长类动物的一种饮食需求，它是一种有效的自由基清除剂，可保护组织免受氧化应激。在这项研究中，为了研究 MEHP 对卵泡发育的毒性作用和 AA 的有益作用，新生小鼠卵巢用不同浓度的 MEHP 处理，添加或不添加 AA 6 天。然后，比较不同组的卵泡构成和氧化状态。结果表明，MEHP 通过增加初级和次级卵泡的百分比并降低卵巢中原始卵泡的百分比来加速原始卵泡的募集。此外，MEHP 通过显着增加丙二醛 (MDA) 浓度以及 GSS 和 SOD1 的表达来诱导卵巢氧化应激。当卵巢与 MEHP 和 AA 共同给药时，卵泡构成正常化，氧化状态显着降低。这些结果表明 AA 改善了 MEHP 诱导的卵巢氧化应激和卵泡失调，这证明了 AA 在 MEHP 暴露的情况下对卵巢保护的临床意义。MEHP 通过显着增加丙二醛 (MDA) 浓度以及 GSS 和 SOD1 的表达来诱导卵巢氧化应激。当卵巢与 MEHP 和 AA 共同给药时，卵泡构成正常化，氧化状态显着降低。这些结果表明 AA 改善了 MEHP 诱导的卵巢氧化应激和卵泡失调，这证明了 AA 在 MEHP 暴露的情况下对卵巢保护的临床意义。MEHP 通过显着增加丙二醛 (MDA) 浓度以及 GSS 和 SOD1 的表达来诱导卵巢氧化应激。当卵巢与 MEHP 和 AA 共同给药时，卵泡结构恢复正常，氧化状态显着降低。这些结果表明 AA 改善了 MEHP 诱导的卵巢氧化应激和卵泡失调，这证明了 AA 在 MEHP 暴露的情况下对卵巢保护的临床意义。