Cells have a rich inner structure that is commonly explored by microscopy. Classical biochemical methods that break apart the cells and fractionate them along a gradient have now gotten a new lease on life through modern methods of mass spectrometry‐based proteomics. Their common principle is to comprehensively measure all the proteins in each of the fractions. The resulting quantitative profile then associates thousands of proteins to their cellular homes. Here, the author recounts how protein correlation profiling, the first such technique, was conceived and how it was applied to answer intricate cell biological questions.

中文翻译：

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通过蛋白质相关性分析进行细胞器映射的起源。

细胞具有丰富的内部结构，通常通过显微镜进行探索。通过基于质谱的现代蛋白质组学方法，将细胞分解并沿梯度分离的经典生化方法现在获得了新的生命。它们的共同原则是综合测量每个馏分中的所有蛋白质。由此产生的定量分析将数以千计的蛋白质与它们的细胞家园联系起来。在这里，作者叙述了蛋白质相关性分析（第一种此类技术）的构想以及如何应用它来回答复杂的细胞生物学问题。