Development and validation of a color spot test method for the presumptive detection of 25-NBOMe compounds,Drug Testing and Analysis

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Development and validation of a color spot test method for the presumptive detection of 25-NBOMe compounds
Drug Testing and Analysis ( IF 2.6 ) Pub Date : 2020-08-03 , DOI: 10.1002/dta.2905
Laura Clancy ₁ , Morgan Philp ₁ , Ronald Shimmon ₁ , Shanlin Fu ₁

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The great increase of new psychoactive substances over the past decade has substantially transformed the illicit drug industry to an ever-changing dynamic market. 25-NBOMe compounds are just one of these new substance groups that pose a public health risk in many countries around the world. These highly potent, hallucinogenic phenethylamines have previously been sold as “legal highs” or “synthetic LSD” and the necessity to rapidly identify their presence is crucial. While there are many laboratory-based analytical methods capable of identifying these compounds, the lack of presumptive test methods indicates the need for a specific and timely test that could be used in the field. Herein we outline the developed chemical spot test that can selectively identify the presence of 25-NBOMe compounds and related analogs through the reaction with a substituted benzoquinone reagent under basic conditions. This test method has been comprehensively validated showing a high level of selectivity, specificity, and precision with only two other illicit substances producing similar positive results as 25-NBOMe and few false-negative results seen. The working limit of detection was determined to be 225 μg and there was no cross-reactivity from potential adulterants of significance. This test has also been shown to work directly with blotter papers containing 25-NBOMe compounds, indicating no interference from this common matrix and the ability to differentiate these compounds from LSD. This method shows a high potential to be translated to a field compatible test that is simple, rapid, and selective for 25-NBOMe compounds.

中文翻译：

用于推定检测 25-NBOMe 化合物的色斑测试方法的开发和验证

过去十年中新型精神活性物质的大量增加已将非法药物行业转变为一个不断变化的动态市场。25-NBOMe 化合物只是在全球许多国家构成公共卫生风险的这些新物质组之一。这些强效、致幻的苯乙胺以前曾作为“合法兴奋剂”或“合成 LSD”出售，因此快速识别它们的存在至关重要。虽然有许多基于实验室的分析方法能够识别这些化合物，但缺乏假定的测试方法表明需要进行可在现场使用的特定和及时的测试。在这里，我们概述了开发的化学点测试，它可以通过在碱性条件下与取代的苯醌试剂反应来选择性地识别 25-NBOMe 化合物和相关类似物的存在。该测试方法已经过全面验证，显示出高水平的选择性、特异性和精确度，只有其他两种非法物质产生与 25-NBOME 相似的阳性结果，并且很少出现假阴性结果。检测的工作限确定为 225 μg，并且不存在来自具有重要意义的潜在掺假物的交叉反应。该测试还被证明可以直接使用含有 25-NBOMe 化合物的吸墨纸，表明这种常见基质没有干扰，并且能够将这些化合物与 LSD 区分开来。

更新日期：2020-08-03

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