Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis.,Acta Crystallographica Section F

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Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis.
Acta Crystallographica Section F ( IF 1.1 ) Pub Date : 2020-08-03 , DOI: 10.1107/s2053230x20009814
Nemanja Vuksanovic ₁ , Xuechen Zhu ₂ , Dante A Serrano ₁ , Vilja Siitonen ₃ , Mikko Metsä-Ketelä ₃ , Charles E Melançon ₂ , Nicholas R Silvaggi ₁

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Proteins belonging to the NTF2‐like superfamily are present in the biosynthetic pathways of numerous polyketide natural products, such as anthracyclins and benzoisochromanequinones. Some have been found to be bona fide polyketide cyclases, but many of them have roles that are currently unknown. Here, the X‐ray crystal structures of three NTF2‐like proteins of unknown function are reported: those of ActVI‐ORFA from Streptomyces coelicolor A3(2) and its homologs Caci_6494, a protein from an uncharacterized biosynthetic cluster in Catenulispora acidiphila, and Aln2 from Streptomyces sp. CM020, a protein in the biosynthetic pathway of alnumycin. The presence of a solvent‐accessible cavity and the conservation of the His/Asp dyad that is characteristic of many polyketide cyclases suggest a potential enzymatic role for these enzymes in polyketide biosynthesis.

中文翻译：

三种非典型 NTF2 样超家族蛋白的结构表征：对聚酮化合物生物合成的影响。

属于 NTF2 样超家族的蛋白质存在于许多聚酮天然产物的生物合成途径中，例如蒽环类和苯并异色满醌。有些已被发现是真正的聚酮化合物环化酶，但其中许多的作用目前尚不清楚。在此，报告了三种功能未知的 NTF2 样蛋白的 X 射线晶体结构：来自天蓝色链霉菌A3(2) 的 ActVI-ORFA 及其同源物 Caci_6494（一种来自嗜酸链孢菌中未表征的生物合成簇的蛋白）和 Aln2来自链霉菌属。CM020，阿努霉素生物合成途径中的一种蛋白质。许多聚酮化合物环化酶所特有的溶剂可及空腔的存在和 His/Asp 二元体的保守性表明这些酶在聚酮化合物生物合成中具有潜在的酶促作用。

更新日期：2020-08-03

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