We investigated the protective effect of Hyperoside (HPS) on liver injury induced by acetaminophen (APAP) in C57 mice. HPS was administered orally for 7 days and APAP was administered orally on the 7th day. Serum and liver samples were then collected for biochemical analyses, histopathology assessments, and metabolomics studies. Metabolites were assessed using a UHPLC‐MS system. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS‐DA) were used to process the data. Pathway analyses were performed using Metaboanalyst 4.0. Western blot and qRT‐PCR were used to determine the protein and mRNA levels, respectively. HPS interacted with active sites in CYP2E1 and caused protein degradation. In conclusion, our results suggested that HPS prevented the oxidative stress‐induced liver injury caused by APAP.

中文翻译：

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代谢组学分析揭示了槲皮素-3-O-半乳糖苷（高糖苷）对乙酰氨基酚引起的小鼠肝损伤的保护作用。

我们调查了Hyperoside（HPS）对对乙酰氨基酚（APAP）诱导的C57小鼠肝损伤的保护作用。HPS口服7天，APAP在第7天口服。然后收集血清和肝样品用于生化分析，组织病理学评估和代谢组学研究。使用UHPLC-MS系统评估代谢产物。主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）用于处理数据。使用Metaboanalyst 4.0进行路径分析。Western blot和qRT-PCR分别用于测定蛋白质和mRNA水平。HPS与CYP2E1中的活性位点相互作用并引起蛋白质降解。总之，我们的结果表明HPS可以预防由APAP引起的氧化应激诱导的肝损伤。