Precise manipulation of gene expression with temporal and spatial control is essential for functional analysis and determining cell lineage relationships in complex biological systems. The cyclic recombinase (Cre)‐loxP system is commonly used for gene manipulation at desired times and places. However, specificity is dependent on the availability of tissue‐ or cell‐specific regulatory elements used in combination with Cre. Here, we present CreLite, an optogenetically controlled Cre system using red light in developing zebrafish embryos.

中文翻译：

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CreLite：使用红光的光遗传学控制的 Cre/loxP 系统。

通过时间和空间控制精确操作基因表达对于功能分析和确定复杂生物系统中的细胞谱系关系至关重要。循环重组酶 (Cre) -lox P 系统通常用于在所需时间和地点进行基因操作。然而，特异性取决于与 Cre 结合使用的组织或细胞特异性调控元件的可用性。在这里，我们展示了 CreLite，这是一种在斑马鱼胚胎发育中使用红光的光遗传学控制的 Cre 系统。