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Orthogonal Test Design for Optimization of the Extraction of Polysaccharides from Inonotus cuticularis and Their Antioxidant Activities
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-08-03 , DOI: 10.1002/cbdv.202000326 Lei Guo 1, 2 , Jia-Yu Ma 1 , Yan-Zhen Ma 2 , Tian-Li Zhang 2 , Sheng-Liu Mao 2 , De-Xian Kong 1 , Yan Hua 1
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-08-03 , DOI: 10.1002/cbdv.202000326 Lei Guo 1, 2 , Jia-Yu Ma 1 , Yan-Zhen Ma 2 , Tian-Li Zhang 2 , Sheng-Liu Mao 2 , De-Xian Kong 1 , Yan Hua 1
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Medical fungi polysaccharides belong to a very important species of biological macromolecules, which are the basic substances that effectively maintain and ensure the normal operation of biological life activities. However, research on extraction and biological activity of Inonotus cuticularis polysaccharides has never been reported. In this study, the optimum yield of Inonotus cuticularis polysaccharides was determined by the orthogonal experimental design. The highest yield of 3.10±0.06 % was obtained with extraction temperature of 80 °C, extraction time of 150 min, and water to raw material ratio of 30 mL/g and repeated twice. After deproteinization for 5 times, the protein removal rate reached 70.10±1.75 %, and the content of polysaccharides and protein were 46.64 and 0.42 %. Infrared spectrometer indicated that Inonotus cuticularis polysaccharides are typical β‐pyranose with characteristic peaks of polysaccharides. Subsequently, the activities of scavenging free radicals for the deproteinated polysaccharides were studied. When the concentration of Inonotus cuticularis polysaccharides was 0.3 mg/mL, the scavenging activities of the sample on DPPH., .OH, ABTS.+ and O2.− reached 83.67±0.27, 65.21±4.82, 43.45±1.36 and 80.28±2.30 %, respectively, and the reducing power reached 0.46±0.01. The IC50 values scavenging DPPH., .OH, ABTS.+ and O2.− were 0.139±0.13, 0.162±0.14, 0.317±0.30 and 0.121±0.10 mg/mL, respectively. Results showed that Inonotus cuticularis polysaccharides present potential stronger antioxidant activities, especially .OH scavenging activity and reducing power. Experimental results could provide research basis of Inonotus cuticularis polysaccharides for further exploitation and utilization.
中文翻译：
木犀多糖提取优化及其抗氧化活性的正交试验设计
药用真菌多糖属于非常重要的生物大分子种类，是有效维持和保证生物生命活动正常运行的基础物质。然而，关于木犀多糖的提取和生物活性的研究尚未见报道。在本研究中，通过正交试验设计确定了木犀多糖的最佳产量。在提取温度为 80 °C、提取时间为 150 min、水与原料比为 30 mL/g 并重复两次时，获得了 3.10±0.06% 的最高收率。5次脱蛋白后，蛋白质去除率达到70.10±1.75%，多糖和蛋白质含量分别为46.64%和0.42%。红外光谱仪表明，木犀多糖是典型的β-吡喃糖，具有多糖特征峰。随后，研究了脱蛋白多糖清除自由基的活性。当木犀多糖浓度为0.3 mg/mL时，样品对DPPH.、.OH、ABTS.+和O2.-的清除活性分别达到83.67±0.27、65.21±4.82、43.45±1.36和80.208%±2.2。 , , 还原力达到 0.46±0.01。清除 DPPH.、.OH、ABTS.+ 和 O2.- 的 IC50 值分别为 0.139±0.13、0.162±0.14、0.317±0.30 和 0.121±0.10 mg/mL。结果表明，木犀多糖具有潜在更强的抗氧化活性，尤其是.OH清除活性和还原能力。
更新日期：2020-08-03
中文翻译：
木犀多糖提取优化及其抗氧化活性的正交试验设计
药用真菌多糖属于非常重要的生物大分子种类，是有效维持和保证生物生命活动正常运行的基础物质。然而，关于木犀多糖的提取和生物活性的研究尚未见报道。在本研究中，通过正交试验设计确定了木犀多糖的最佳产量。在提取温度为 80 °C、提取时间为 150 min、水与原料比为 30 mL/g 并重复两次时，获得了 3.10±0.06% 的最高收率。5次脱蛋白后，蛋白质去除率达到70.10±1.75%，多糖和蛋白质含量分别为46.64%和0.42%。红外光谱仪表明，木犀多糖是典型的β-吡喃糖，具有多糖特征峰。随后，研究了脱蛋白多糖清除自由基的活性。当木犀多糖浓度为0.3 mg/mL时，样品对DPPH.、.OH、ABTS.+和O2.-的清除活性分别达到83.67±0.27、65.21±4.82、43.45±1.36和80.208%±2.2。 , , 还原力达到 0.46±0.01。清除 DPPH.、.OH、ABTS.+ 和 O2.- 的 IC50 值分别为 0.139±0.13、0.162±0.14、0.317±0.30 和 0.121±0.10 mg/mL。结果表明，木犀多糖具有潜在更强的抗氧化活性，尤其是.OH清除活性和还原能力。
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