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J-Aggregate-Based FRET Monitoring of Drug Release from Polymer Nanoparticles with High Drug Loading.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-08-02 , DOI: 10.1002/anie.202008018
Yun Liu 1 , Guangze Yang 1 , Song Jin 1 , Run Zhang 1 , Peng Chen 2 , Tengjisi 1 , Lianzhou Wang 2 , Dong Chen 3 , David A Weitz 4 , Chun-Xia Zhao 1
Affiliation  

Understanding drug‐release kinetics is critical for the development of drug‐loaded nanoparticles. We developed a J‐aggregate‐based Förster‐resonance energy‐transfer (FRET) method to investigate the release of novel high‐drug‐loading (50 wt %) nanoparticles in comparison with low‐drug‐loading (0.5 wt %) nanoparticles. Single‐dye‐loaded nanoparticles form J‐aggregates because of the high dye‐loading (50 wt %), resulting in a large red‐shift (≈110 nm) in the fluorescence spectrum. Dual‐dye‐loaded nanoparticles with high dye‐loading using FRET pairs exhibited not only FRET but also a J‐aggregate red‐shift (116 nm). Using this J‐aggregate‐based FRET method, dye‐core–polymer‐shell nanoparticles showed two release processes intracellularly: the dissolution of the dye aggregates into dye molecules and the release of the dye molecules from the polymer shell. Also, the high‐dye‐loading nanoparticles (50 wt %) exhibited a slow release kinetics in serum and relatively quick release in cells, demonstrating their great potential in drug delivery.

中文翻译:

基于J聚合的FRET监测具有高载药量的聚合物纳米颗粒中药物的释放。

了解药物释放动力学对于载药纳米颗粒的开发至关重要。我们开发了一种基于J聚集体的Förster共振能量转移(FRET)方法,以研究新型的高载药量(50 wt%)纳米颗粒与低载药量(0.5 wt%)纳米颗粒的释放。由于高染料负载量(50 wt%),单染料负载的纳米颗粒形成J聚集体,导致荧光光谱中出现大的红移(≈110nm)。使用FRET对具有高染料负载量的双染料负载纳米颗粒不仅表现出FRET,而且表现出J聚集的红移(116 nm)。使用这种基于J聚集体的FRET方法,染料核聚合物壳纳米粒子在细胞内显示出两个释放过程:染料聚集到染料分子中,染料分子从聚合物壳中释放出来。同样,高染料含量的纳米粒子(50 wt%)在血清中表现出缓慢的释放动力学,而在细胞中表现出相对快速的释放,证明了其在药物递送方面的巨大潜力。
更新日期:2020-08-02
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