Four different pesticide residues used in barley planting were selected to investigate their effects on simulated beer brewing. The influences were found to be different by varied agricultural chemicals. Among the four types of pesticides, at 25 μg/mL, triadimefon or carbendazim barely affected the brewing progress. However, ethametsulfuron-methyl and carbaryl (15 μg/mL and 2.5 μg/mL, respectively) exhibited slightly inhibition on saccharification and significantly negative impacts on yeast growth and alcohol fermentation. After pretreated by 50 μL carbaryl-degrading enzyme with the K_cat value of 2.12 s⁻¹ at 30°C for 90 min, the negative influence on simulated beer brewing brought by carbaryl can be eliminated in the fermentation system containing 2.5 μg/mL carbaryl. The efficiency of ethanol fermentation was improved, and the removal rate of carbaryl in the brewing system was greatly accelerated. Taken together, this study suggested a potential method for solving the fermentation inhibition by pesticide residues.

选择了大麦种植中使用的四种不同农药残留，以研究它们对模拟啤酒酿造的影响。发现各种农用化学品的影响是不同的。在四种类型的农药（25μg/ mL）中，三唑酮或多菌灵对酿造进度几乎没有影响。然而，乙胺磺隆和甲萘威（分别为15μg/ mL和2.5μg/ mL）对糖化作用表现出轻微的抑制作用，并对酵母的生长和酒精发酵产生显着的负面影响。用50μL甲氧萘降解酶预处理后的K _cat值为2.12 s ^-1在30°C下持续90分钟，可以在含有2.5μg/ mL西维因的发酵系统中消除西维因对模拟啤酒酿造的负面影响。提高了乙醇发酵效率，大大提高了酿造系统中甲硫基的去除率。两者合计，这项研究提出了解决农药残留发酵抑制的潜在方法。