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Myco-valorization approach using entrapped Myrothecium verrucaria ITCC-8447 on synthetic and natural support via column bioreactor for the detoxification and degradation of anthraquinone dyes
International Biodeterioration & Biodegradation ( IF 4.1 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.ibiod.2020.105052
Komal Agrawal , Pradeep Verma

Abstract Myco-valorization (Myco-Valtn) concept was explored using Myrothecium verrucaria ITCC-8447 via column bioreactor to remove two anthraquinone (ARq) dyes. Among the three myco-valtn approaches free cell dependent enabled more than 80% removal of Anthraquinone violet R (AVR) and Alizarin cyanine green (ACG) after 48 h at 30 ± 2 °C. Enzymatic treatment removed more than 50% AVR and ACG after 10 min. However, synthetic and natural support entrapment on Scotch-Brite® (SB) and Luffa cylindrica (LC) enabled more than 80% removal in SB-ACG and LC-ACG and more than 60% in SB-AVR and LC-AVR in 6th cycle. The regenerated column enhanced the removal of dye and allowed the column to run for multiple cycles. Further after FTIR XRD and LCMS Q-TOF, analysis it was observed that ACG and AVR were completely degraded and laccase played the key roles in the degradation of ACG and AVR and the degraded metabolites of anthraquinone dyes (ACG and AVR) exhibited much less toxicity than the parent dye using phytotoxicity assays.

中文翻译:

通过柱式生物反应器在合成和天然载体上使用包埋的疣孢漆霉 ITCC-8447 的 Myco-valorization 方法对蒽醌染料进行解毒和降解

摘要 Myco-valorization (Myco-Valtn) 概念是通过柱状生物反应器使用疣状漆斑菌 ITCC-8447 去除两种蒽醌 (ARq) 染料进行的。在三种 myco-valtn 方法中,游离细胞依赖性能够在 30±2°C 下 48 小时后去除 80% 以上的蒽醌紫 R (AVR) 和茜素青绿 (ACG)。10 分钟后酶处理去除了超过 50% 的 AVR 和 ACG。然而,在 Scotch-Brite® (SB) 和 Luffa cylindrica (LC) 上的合成和天然载体截留使 SB-ACG 和 LC-ACG 的去除率超过 80%,SB-AVR 和 LC-AVR 的去除率超过 60%循环。再生柱增强了染料的去除并允许柱运行多个循环。进一步在 FTIR XRD 和 LCMS Q-TOF 之后,
更新日期:2020-09-01
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