Soil samples of 0–10 cm and subsurface depths of 10–20 cm were collected from the Phragmites australis soil in the Liaohe Estuary, and different salinities (CK, 0.5%, 1.0%, 1.5%, and 2.0%) were prepared in the laboratory under simulated water flooding; soil samples of two depths were added for cultivation under anaerobic conditions, measuring Hg and MeHg contents in soil samples. The results showed that with the increase of salinity, the content of Hg and MeHg in the soil decreased; when the salinity was 2.0%, salinity significantly inhibited the occurrence of mercury methylation. The mass ratio of MeHg in the soils at the depths of 0–10 cm and 10–20 cm increased with the increase of the culture time. It showed that the MeHg mass ratio increased rapidly on the 15th day but decreased significantly on the 25th day; there was no significant correlation between the soil Hg mass ratio and pH and organic matter. The soil Hg mass ratio at a depth of 10–20 cm was negative to SRB correlation relationship; according to the linear relationship, when the SRB content was large, it was not conducive to provide more mercury ions for the occurrence of mercury methylation. There was no significant negative correlation between Hg and MeHg in different depths of soil.

中文翻译：

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芦苇土壤汞甲基化及其影响因素研究。

从芦苇中收集了0–10 cm的土壤样品和10–20 cm的地下深度在模拟注水条件下，在实验室中制备了辽河口的土壤，并制备了不同的盐度（CK，0.5％，1.0％，1.5％和2.0％）；加入两个深度的土壤样品，在厌氧条件下进行培养，测量土壤样品中的Hg和MeHg含量。结果表明，随着盐度的增加，土壤中Hg和MeHg的含量降低。当盐度为2.0％时，盐度显着抑制了汞甲基化的发生。随着培养时间的增加，土壤中0–10 cm和10–20 cm深度处MeHg的质量比增加。结果表明，甲基汞质量比在第15天迅速增加，但在第25天显着下降；土壤汞质量比与pH和有机质之间无显着相关性。10–20 cm深度的土壤汞质量比与SRB相关关系呈负相关。根据线性关系，当SRB含量较大时，不利于提供更多的汞离子用于发生汞甲基化。在不同土壤深度下，汞和甲基汞之间无显着负相关。