Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access soil moisture and bigger seeds could improve emergence and stand establishment by providing the energy necessary for seedling elongation. AHL (AT-Hook Containing, NuclearLocalized) genes play an important role in seedling growth and development. AHL proteins contain two structural units, the DNA-binding AT-hook motif and the Plant and Prokaryote Conserved (PPC) domain, required for protein–protein interactions. Our previous studies demonstrate that AtAHL29/SOB3 (Suppressorof phytochromeB-4 #3) regulates seedling development in Arabidopsis (Arabidopsis thaliana). Activation-tagged overexpression of AtSOB3 (Atsob3-D) represses the long-hypocotyl phenotype of an Arabidopsis phytochrome B mutant. In contrast, overexpression of the Atsob3-6 variant (Atsob3-6-OX), with a non-functional AT-hook, confers a long-hypocotyl phenotype. In this study, we demonstrate the role of Atsob3-D and Atsob3-6-OX in modulating seed size and hypocotyl length in the brassicas Arabidopsis and Camelina. In Arabidopsis, Atsob3-D reduces seed weight whereas Atsob3-6-OX increases seed weight and size when compared to the wild type. Similarly, Atsob3-6-OX transgenic Camelina seedlings are taller than the wild type, and produce larger and heavier seeds. These larger Atsob3-6-OX Camelina seeds also confer better emergence in deep-soil planting when compared to the wild type. Taken together, Atsob3-6-OX increases seed size, seed weight, seedling hypocotyl length and stand establishment in the oilseed crop Camelina.

在低降水量农业地区，建立苗木是影响作物产量的关键因素。对于需要播种浅的小种子作物，例如山茶（Camelina sativa）和油菜（芸苔）（Brassica napus），尤其如此。需要更深的播种，以便种子可以提供土壤水分，更大的种子可以通过提供幼苗延长所需的能量来改善出苗率和林分建立。AHL（甲Ť - ħ OOK含有核大号ocalized）基因在幼苗生长发育中起着重要作用。AHL蛋白包含两个结构单元中，DNA结合AT-钩基序和P兰特和P rokaryote Ç onserved（PPC）域，蛋白质-蛋白质相互作用必需的。我们以前的研究表明，AtAHL29 / SOB3（小号uppressor Ø ˚F光敏色素乙- 4＃3）调节在幼苗发育拟南芥（拟南芥）。激活标记的AtSOB3（Atsob3 - D）的过表达抑制了拟南芥的长下胚轴表型拟南芥植物色素B突变体。相反，过量表达Atsob3 - 6变体（Atsob3 - 6 - OX），具有非功能性AT-钩，赋予长的下胚轴表型。在这项研究中，我们展示的作用Atsob3 - d和Atsob3 - 6 - OX在芸苔属植物调节种子大小和下胚轴长拟南芥和亚麻。在拟南芥，Atsob3 - d降低的种子重量，而Atsob3 - 6 -与野生型相比，OX增加了种子的重量和大小。类似地，Atsob3 - 6 - OX转基因亚麻籽苗比野生型高，并产生较大和较重的种子。这些较大的Atsob3 - 6 - OX亚麻种子也胙在深土播种出苗较好时相比，野生型。两者合计，Atsob3 - 6 - OX增加种子大小，种子重量，幼苗下胚轴长度和油料种子作物站建立亚麻荠。