Antibiotics, which can be used as veterinary drugs, are widely used in the prevention and treatment of infectious diseases for animals. However, overuse of antibiotics had caused serious problems on food contamination and human harm. For control such public issues, several of techniques have been in recent years. Ratiometric fluorescent (RF) technique, as one of the most promising strategies for quantitatively evaluated analytes, had been extensively developed for the readily measurements on the two different fluorescent emission intensities. In this review, the construction strategies for recent RF sensors will be mainly focused on. Meanwhile, the recent advances and new tendencies for detection of antibiotics based on RF technique shall be introduced. Finally, outlooks on the opportunities and challenges for quantitative fluorescence sensing on antibiotics will be summarized.

抗生素可作为兽药，广泛用于动物传染病的防治。然而，过度使用抗生素已造成严重的食品污染和人类伤害问题。为了控制此类公共问题，近年来已经采用了几种技术。比率荧光 (RF) 技术作为定量评估分析物的最有前途的策略之一，已被广泛开发用于轻松测量两种不同的荧光发射强度。在本次审查中，将主要关注近期射频传感器的构建策略。同时介绍了基于射频技术检测抗生素的最新进展和新趋势。最后，