A Gram-positive, endospore-forming, rod-shaped bacterium with a single flagellum, and a motile strain, designated CX253, was isolated from bioaerosols. The isolate is facultatively anaerobic, is able to grow at 25–45 ℃ (optimum 37 ℃) and pH 6.5–10.0 (optimum 7.5), and can tolerate up to 5.0% NaCl (w/v) under aerobic conditions. The diagnostic diamino acid in the cell wall of strain CX253T is meso-diaminopimelic acid, while major isoprenoid quinone is menaquinone 6 (MK-6) along with a smaller amount of MK-7 (20%). The polar lipid profile is composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids and glycolipids. The major cellular fatty acid is iso-C15:0 and anteiso-C15:0. Phylogenetic analysis based on 16S rRNA gene and genome sequence grouped strain CX253T into the genus Bacillus. The strain was most closely related to Bacillus thermotolerans CCTCC AB 2012108 T by comparison of 16S rRNA gene sequence (97.2% similarity) and to Bacillus wudalianchiensis CCTCC AB 2015266 T by comparison of gyrB gene sequence (80.1% similarity). The draft genome of strain CX253T comprised 3,929,195 bp with a G + C content of 43.3 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain CX253T and phylogenetically related Bacillus species were lower than 95% and 70%, respectively. Thus, the polyphasic evidence generated through phenotypic, chemotaxonomic and genomic methods confirmed that strain CX253T (= GDMCC 1.1608 T = KACC 21318 T) was a novel species of the genus Bacillus, for which the name Bacillus aerolatus sp. nov. is proposed.

中文翻译：

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气化芽孢杆菌 nov.，芽孢杆菌属的新成员，从​​学校操场的生物气溶胶中分离出来

从生物气溶胶中分离出一种革兰氏阳性、形成内生孢子、杆状的细菌，具有单个鞭毛和运动菌株，命名为 CX253。该分离株具有兼性厌氧性，能够在 25-45 ℃（最佳 37 ℃）和 pH 6.5-10.0（最佳 7.5）下生长，在有氧条件下可耐受高达 5.0% NaCl (w/v)。CX253T 菌株细胞壁中的诊断二氨基酸是内消旋二氨基庚二酸，而主要的类异戊二烯醌是甲基萘醌 6 (MK-6) 以及少量的 MK-7 (20%)。极性脂质谱由二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂和糖脂组成。主要的细胞脂肪酸是异 C15:0 和反异 C15:0。基于16S rRNA基因和基因组序列的系统发育分析将菌株CX253T归入芽孢杆菌属。通过16S rRNA基因序列的比较（97.2%的相似性）和gyrB基因序列的比较（80.1%的相似性），该菌株与耐热芽孢杆菌CCTCC AB 2012108 T最密切相关（97.2%的相似性）和与五大连池芽孢杆菌CCTCC AB 2015266 T的最密切相关。菌株 CX253T 的基因组草图包含 3,929,195 bp，G + C 含量为 43.3 mol%。CX253T 菌株与系统发育相关芽孢杆菌属物种之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别低于 95% 和 70%。因此，通过表型、化学分类学和基因组方法产生的多相证据证实菌株 CX253T（= GDMCC 1.1608 T = KACC 21318 T）是芽孢杆菌属的一个新物种，其名称为 Bacillus aerolatus sp。十一月 被提议。