The aim of this research was to gauge the alternations in the lipid peroxidation and antioxidative enzyme activity in two cultivars (cv. RGS003 and cv. Sarigol) of canola under drought stress and drought tolerance amelioration by penconazole (PEN) and calcium (Ca). Plants were treated with different polyethylene glycol (PEG) concentrations (0, 5, 10 and 15%) without or with PEN (15 mg L^–1) and Ca (15 mM). The Ca treatment prevented the negative effects of drought on fresh weight (FW) in RGS003 and Sarigol at 5 and 15% PEG respectively. Ca and PEN/Ca treatments caused significant induction in the proline content in Sarigol at 15% PEG; the latter treatment was accompanied by higher glycine betaine (GB), lower malondialdehyde (MDA) and growth recovery. Hydrogen peroxide (HO₂) content in Sarigol was proportional to the severity of drought stress and all PEN, Ca and PEN/Ca treatments significantly reduced the H₂O₂ content. PEN and PEN/Ca caused alleviation of the drought-induced oxidative stress in RGS003. RGS003 cultivar exhibited significantly higher antioxidative enzymes activity at most levels of drought, which could lead to its drought tolerance and lower MDA content. In contrast to that of Sarigol, the activity of catalase and superoxide dismutase (SOD) increased with Ca and PEN/Ca treatments in RGS003 under low stress. The application of PEN and Ca induced significantly P5CS and SOD expression in RGS003 under drought stress after 24 h. Overall, these data demonstrated that PEN and Ca have the ability to enhance the tolerance against the drought stress in canola plants.

这项研究的目的是评估油菜在干旱胁迫下的两个油菜品种（RGS003和Sarigol品种）的脂质过氧化和抗氧化酶活性的变化，并通过戊康唑（PEN）和钙（Ca）改善耐旱性。用不同浓度的聚乙二醇（PEG）（0、5、10和15％）处理植物，不使用PEN（15 mg L ^–1）和Ca（15 mM）。钙处理可防止干旱对分别含5％和15％PEG的RGS003和Sarigol中的鲜重（FW）产生不利影响。Ca和PEN / Ca处理在15％PEG时显着诱导了Sarigol中脯氨酸的含量。后一种处理伴随着更高的甘氨酸甜菜碱（GB），更低的丙二醛（MDA）和生长恢复。过氧化氢（HO ₂Sarigol中的含量与干旱胁迫的严重程度成正比，所有PEN，Ca和PEN / Ca处理均显着降低了H ₂ O ₂含量。PEN和PEN / Ca减轻了RGS003干旱引起的氧化应激。RGS003品种在大多数干旱水平下均表现出较高的抗氧化酶活性，这可能导致其耐旱性和MDA含量降低。与Sarigol相比，RGS003在低胁迫下，Ca和PEN / Ca处理可增加过氧化氢酶和超氧化物歧化酶（SOD）的活性。PEN和Ca的应用显着诱导了P5CS和SOD24 h后干旱胁迫下RGS003的表达。总体而言，这些数据表明PEN和Ca具有增强油菜籽植物对干旱胁迫的耐受性的能力。