Bradyrhizobium elkanii BLY3-8 does not form nodules on the roots of Rj3-genotype soybean (cultivar D-51). This is a cultivar-specific nodulation restriction. The genes A6X20_40975 and A6X20_41030 in strain BLY3-8 were predicted to encode the transcriptional activator and apparatus of the type III secretion system (T3SS) (the proteins TtsI and RhcJ), respectively. Mutants disrupted in these genes overcame the nodulation restriction. These results suggest that an effector injected via T3SS into Rj3-genotype soybean is involved in nodulation restriction by Rj3-genotype soybean.

中文翻译：

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埃氏慢生根瘤菌 BLY3-8 III 型分泌系统被破坏的突变体克服了 Rj3 基因型大豆的结瘤限制


埃尔卡慢生根瘤菌 BLY3-8 不会在 Rj3 基因型大豆（品种 D-51）的根部形成根瘤。这是品种特定的结瘤限制。菌株 BLY3-8 中的基因 A6X20_40975 和 A6X20_41030 预计分别编码 III 型分泌系统 (T3SS) 的转录激活子和装置（蛋白质 TtsI 和 RhcJ）。这些基因被破坏的突变体克服了结瘤限制。这些结果表明，通过 T3SS 注射到 Rj3 基因型大豆中的效应子参与了 Rj3 基因型大豆的结瘤限制。