To identify exoelectrogens involved in the generation of electricity from complex organic matter in coastal sediment (CS) microbial fuel cells (MFCs), MFCs were inoculated with CS obtained from tidal flats and estuaries in the Tokyo bay and supplemented with starch, peptone, and fish extract as substrates. Power output was dependent on the CS used as inocula and ranged between 100 and 600 mW m–2 (based on the projected area of the anode). Analyses of anode microbiomes using 16S rRNA gene amplicons revealed that the read abundance of some bacteria, including those related to Shewanella algae, positively correlated with power outputs from MFCs. Some fermentative bacteria were also detected as major populations in anode microbiomes. A bacterial strain related to S. algae was isolated from MFC using an electrode plate-culture device, and pure-culture experiments demonstrated that this strain exhibited the ability to generate electricity from organic acids, including acetate. These results suggest that acetate-oxidizing S. algae relatives generate electricity from fermentation products in CS-MFCs that decompose complex organic matter.

中文翻译：

---

能够从醋酸发电的希瓦氏藻类亲戚有助于沿海沉积物微生物燃料电池处理复杂的有机物

为了鉴定沿海沉积物 (CS) 微生物燃料电池 (MFC) 中复杂有机物发电所涉及的外电原，MFC 接种了从东京湾的潮滩和河口获得的 CS，并辅以淀粉、蛋白胨和鱼类提取物作为底物。功率输出取决于用作接种物的 CS，范围在 100 到 600 mW m-2 之间（基于阳极的投影面积）。使用 16S rRNA 基因扩增子对阳极微生物组的分析表明，一些细菌（包括与希瓦氏藻相关的细菌）的读数丰度与 MFC 的功率输出呈正相关。一些发酵细菌也被检测为阳极微生物组中的主要种群。使用电极板培养装置从 MFC 中分离出与 S. 藻类相关的细菌菌株，纯培养实验表明，该菌株具有利用有机酸（包括醋酸盐）发电的能力。这些结果表明，醋酸盐氧化性 S. 藻类亲属通过 CS-MFC 中的发酵产物发电，可分解复杂的有机物。